Baskurt O K
Department of Physiology, Akdeniz University Faculty of Medicine, Turkey.
Biorheology. 1996 Mar-Apr;33(2):169-79. doi: 10.1016/0006-355X(96)00014-5.
The Cell Transit Analyzer (CTA) is now being used widely in clinical hemorheology. Most of the data obtained by CTA are limited to human blood, although the CTA has an important potential to be used in experimental studies on animal models. However, behavior of red blood cells (RBC) from various species might be different in CTA. Eight parameters reflecting different aspects of cell passage through pores with 5 microns diameter and 15 microns length were determined or human, guinea pig, dog, rabbit, rat, mouse and sheep RBC, together with instrument precision and biological variation. These parameters have a wide range when measured in different species and correlate with cell volume. Sensitivity of these parameters to the glutaraldehyde-induced alterations in RBC deformability was not same for different laboratory mammals. The main reason for this difference seems to be related to the cell size and thus sensitivity might be significantly limited if 5 microns pore-size filters are used to test the smaller RBC. The results of this study may help in designing experimental studies on laboratory mammals using the CTA.
细胞转运分析仪（CTA）目前在临床血液流变学中得到广泛应用。尽管CTA在动物模型实验研究中具有重要的应用潜力，但通过CTA获得的大多数数据仅限于人体血液。然而，不同物种的红细胞（RBC）在CTA中的行为可能会有所不同。测定了反映细胞通过直径5微米、长度15微米的孔的不同方面的八个参数，这些参数针对人类、豚鼠、狗、兔子、大鼠、小鼠和绵羊的红细胞进行测定，同时还测定了仪器精度和生物学变异。这些参数在不同物种中测量时范围很广，并且与细胞体积相关。对于不同的实验哺乳动物，这些参数对戊二醛诱导的RBC变形性改变的敏感性并不相同。这种差异的主要原因似乎与细胞大小有关，因此，如果使用5微米孔径的过滤器来测试较小的RBC，敏感性可能会受到显著限制。本研究结果可能有助于设计使用CTA对实验哺乳动物进行的实验研究。
