Patel R T, Pall A A, Adu D, Keighley M R
Department of Surgery, Queen Elizabeth Hospital, Birmingham, UK.
Eur J Gastroenterol Hepatol. 1995 Nov;7(11):1037-41. doi: 10.1097/00042737-199511000-00005.
To determine levels of soluble forms of the cell adhesion molecules (CAM), ICAM-1, E-Selectin and VCAM-1 in relation to prevalence, treatment and disease activity in inflammatory bowel disease.
Plasma was obtained from patients with ulcerative colitis (n = 49), patients with ulcerative colitis who had undergone restorative proctocolectomy (n = 32, eight of whom had a clinical pouchitis), Crohn's disease patients (n = 34) and 24 healthy controls.
Plasma soluble ICAM-1 levels [medians (ranges in ng/ml)] were significantly higher in patients with active ulcerative colitis [270 (90-510)], pouchitis [415 (310-670)] and active Crohn's disease [305 (200-630)] than in those with inactive ulcerative colitis [225 (140-425), P = 0.031], non-inflamed ileoanal pouch [260 (140-380), P = 0.0004] and inactive Crohn's disease [245 (90-520), P = 0.045], respectively, and controls. The soluble E-Selectin levels were also significantly higher in patients with active ulcerative colitis [55 (40-140)], pouchitis [90 (45-145)], and active Crohn's disease [78 (30-115)] than in those with inactive ulcerative colitis [45 (20-80, P = 0.003], non-inflamed ileoanal pouch [45 (20-90), P = 0.001] and inactive Crohn's disease [48 (25-90, P = 0.020], respectively, and controls.
The present study suggests that increased levels of soluble ICAM-1 and soluble E-Selectin occur during active inflammatory bowel disease and pouchitis, which may be used as sensitive markers of continuing inflammation.
测定细胞黏附分子（CAM）的可溶性形式，即细胞间黏附分子-1（ICAM-1）、E-选择素和血管细胞黏附分子-1（VCAM-1）的水平，并探讨其与炎症性肠病的患病率、治疗及疾病活动度之间的关系。
采集溃疡性结肠炎患者（n = 49）、接受结直肠全切除回肠储袋肛管吻合术的溃疡性结肠炎患者（n = 32，其中8例有临床袋炎）、克罗恩病患者（n = 34）以及24名健康对照者的血浆。
活动期溃疡性结肠炎患者[270（90 - 510）]、袋炎患者[415（310 - 670）]和活动期克罗恩病患者[305（200 - 630）]的血浆可溶性ICAM-1水平[中位数（单位：ng/ml）]显著高于非活动期溃疡性结肠炎患者[225（140 - 425），P = 0.031]、无炎症的回肠储袋[260（140 - 380），P = 0.0004]和非活动期克罗恩病患者[245（90 - 520），P = 0.045]以及健康对照者。活动期溃疡性结肠炎患者[55（40 - 140）]、袋炎患者[90（45 - 145）]和活动期克罗恩病患者[78（30 - 115）]的可溶性E-选择素水平也显著高于非活动期溃疡性结肠炎患者[45（20 - 80，P = 0.003]、无炎症的回肠储袋[45（20 - 90），P = 0.001]和非活动期克罗恩病患者[48（25 - 90，P = 0.020]以及健康对照者。
本研究表明，在活动期炎症性肠病和袋炎期间，可溶性ICAM-1和可溶性E-选择素水平升高，这可能用作持续炎症的敏感标志物。
