Pooley N, Ghosh L, Sharon P
Department of Medicine, University of Illinois at Chicago 60612-7323.
Dig Dis Sci. 1995 Jan;40(1):219-25. doi: 10.1007/BF02063969.
In present study, we investigated if inflammatory mediators secreted by the inflamed colonic mucosa from patients with Crohn's disease and ulcerative colitis had the ability to up-regulate the expression of two adhesion molecules, E-selectin and intercellular adhesion molecule-1. Organ culture techniques and enzyme-linked immunoassays were used to quantify these up-regulations in human umbilical vein endothelial cells. Our results show that, in Crohn's disease patients, the expression of E-selectin was up-regulated 5.5-fold over control values and intercellular adhesion molecule-1 expression was increased 2.4-fold. In ulcerative colitis patients, E-selectin expression was up-regulated twofold over controls with only a 1.5-fold increase in intercellular adhesion molecule-1 expression. Histologically, there was no difference in the degree of inflammation between the two disease groups. Sulfasalazine, in a dose-dependent manner, inhibited E-selectin expression up to 58% and intercellular adhesion molecule-1 up to 62% when stimulated by lipopolysaccharide. The up-regulation of E-selectin and intercellular adhesion molecule-1 may play an important role in mediating the inflammatory process in inflammatory bowel disease. The observed difference between Crohn's disease and ulcerative colitis may reflect differences in inflammatory cell infiltrates or the histopathological differences between the two diseases.
在本研究中，我们调查了克罗恩病和溃疡性结肠炎患者发炎的结肠黏膜分泌的炎症介质是否有能力上调两种黏附分子E-选择素和细胞间黏附分子-1的表达。采用器官培养技术和酶联免疫测定法来量化人脐静脉内皮细胞中的这些上调情况。我们的结果显示，在克罗恩病患者中，E-选择素的表达比对照值上调了5.5倍，细胞间黏附分子-1的表达增加了2.4倍。在溃疡性结肠炎患者中，E-选择素的表达比对照上调了两倍，而细胞间黏附分子-1的表达仅增加了1.5倍。组织学上，两个疾病组之间的炎症程度没有差异。柳氮磺胺吡啶以剂量依赖的方式，在受到脂多糖刺激时，将E-选择素的表达抑制高达58%，将细胞间黏附分子-1的表达抑制高达62%。E-选择素和细胞间黏附分子-1的上调可能在介导炎症性肠病的炎症过程中起重要作用。观察到的克罗恩病和溃疡性结肠炎之间的差异可能反映了炎症细胞浸润的差异或这两种疾病之间的组织病理学差异。
