A general assay for restriction endonucleases and other DNA-modifying enzymes with plasmid substrates.
作者信息
Vipond I B, Baldwin G S, Oram M, Erskine S G, Wentzell L M, Szczelkun M D, Nobbs T J, Halford S E
机构信息
Department of Biochemistry, Centre for Molecular Recognition, University of Bristol, UK.
出版信息
Mol Biotechnol. 1995 Dec;4(3):259-68. doi: 10.1007/BF02779019.
PMID:8680932
Abstract
A procedure for measuring the activities of enzymes that alter the covalent structure of DNA is described. The assay utilizes covalently closed circles of DNA as the substrate and yields quantitative data on the fraction of this DNA converted to both open-circle and linear forms.
摘要
Zotero 插件