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A general assay for restriction endonucleases and other DNA-modifying enzymes with plasmid substrates.

作者信息

Vipond I B, Baldwin G S, Oram M, Erskine S G, Wentzell L M, Szczelkun M D, Nobbs T J, Halford S E

机构信息

Department of Biochemistry, Centre for Molecular Recognition, University of Bristol, UK.

出版信息

Mol Biotechnol. 1995 Dec;4(3):259-68. doi: 10.1007/BF02779019.

DOI:10.1007/BF02779019
PMID:8680932
Abstract

A procedure for measuring the activities of enzymes that alter the covalent structure of DNA is described. The assay utilizes covalently closed circles of DNA as the substrate and yields quantitative data on the fraction of this DNA converted to both open-circle and linear forms.

摘要

相似文献

1
A general assay for restriction endonucleases and other DNA-modifying enzymes with plasmid substrates.
Mol Biotechnol. 1995 Dec;4(3):259-68. doi: 10.1007/BF02779019.
2
The EcoRI restriction endonuclease, covalently closed DNA and ethidium bromide.EcoRI 限制性内切酶、共价闭合 DNA 和溴化乙锭。
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3
A method for the deletion of restriction sites in bacterial plasmid deoxyribonucleic acid.一种用于删除细菌质粒脱氧核糖核酸中限制酶切位点的方法。
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4
The reactions of the EcoRi and other restriction endonucleases.EcoRi及其他限制性内切酶的反应
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5
[Reaction of endonuclease EcoRI with DNA of the plasmid ColEI].
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ClpXP protease targets long-lived DNA translocation states of a helicase-like motor to cause restriction alleviation.

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Vaccinia DNA topoisomerase I: single-turnover and steady-state kinetic analysis of the DNA strand cleavage and ligation reactions.痘苗病毒DNA拓扑异构酶I：DNA链切割与连接反应的单轮和稳态动力学分析
Biochemistry. 1994 Jan 11;33(1):327-39. doi: 10.1021/bi00167a043.
2
Rapid reaction analysis of the catalytic cycle of the EcoRV restriction endonuclease.
Biochemistry. 1995 Jan 17;34(2):705-14. doi: 10.1021/bi00002a038.
3
Trans-complementable copy-number mutants of plasmid ColE1.质粒ColE1的反式可互补拷贝数突变体
ClpXP蛋白酶靶向解旋酶样马达的长寿命DNA易位状态以减轻限制。
Nucleic Acids Res. 2014 Oct 29;42(19):12082-91. doi: 10.1093/nar/gku851. Epub 2014 Sep 26.
4
The Type ISP Restriction-Modification enzymes LlaBIII and LlaGI use a translocation-collision mechanism to cleave non-specific DNA distant from their recognition sites.LlaBIII 和 LlaGI 型 ISR 限制修饰酶使用易位-碰撞机制切割与其识别位点远离的非特异性 DNA。
Nucleic Acids Res. 2013 Jan;41(2):1071-80. doi: 10.1093/nar/gks1209. Epub 2012 Dec 7.
5
DNA cleavage by Type ISP Restriction-Modification enzymes is initially targeted to the 3'-5' strand.I 型 DNA 限制性内切酶的 DNA 切割作用最初靶向于 3'-5'链。
Nucleic Acids Res. 2013 Jan;41(2):1081-90. doi: 10.1093/nar/gks1210. Epub 2012 Dec 5.
6
DNA cleavage site selection by Type III restriction enzymes provides evidence for head-on protein collisions following 1D bidirectional motion.III 型限制酶对 DNA 切割位点的选择为沿 1D 双向运动的蛋白迎面相撞提供了证据。
Nucleic Acids Res. 2011 Oct;39(18):8042-51. doi: 10.1093/nar/gkr502. Epub 2011 Jun 30.
7
Recycling of protein subunits during DNA translocation and cleavage by Type I restriction-modification enzymes.I 型限制修饰酶在 DNA 转位和切割过程中对蛋白质亚基的回收。
Nucleic Acids Res. 2011 Sep 1;39(17):7656-66. doi: 10.1093/nar/gkr479. Epub 2011 Jun 28.
8
Concerted action at eight phosphodiester bonds by the BcgI restriction endonuclease.八个磷酸二酯键的协同作用由 BcgI 限制内切酶完成。
Nucleic Acids Res. 2011 Sep 1;39(17):7630-40. doi: 10.1093/nar/gkr453. Epub 2011 Jun 7.
9
DNA cleavage and methylation specificity of the single polypeptide restriction-modification enzyme LlaGI.LlaGI 单多肽限制修饰酶的 DNA 切割和甲基化特异性。
Nucleic Acids Res. 2009 Nov;37(21):7206-18. doi: 10.1093/nar/gkp790.
10
Motor step size and ATP coupling efficiency of the dsDNA translocase EcoR124I.双链DNA转位酶EcoR124I的运动步长和ATP偶联效率
EMBO J. 2008 May 7;27(9):1388-98. doi: 10.1038/emboj.2008.69. Epub 2008 Apr 3.
Nature. 1980 Jan 10;283(5743):216-8. doi: 10.1038/283216a0.
4
Single turnovers of the EcoRI restriction endonuclease.EcoRI 限制性内切酶的单轮反应。
Biochem J. 1983 May 1;211(2):405-15. doi: 10.1042/bj2110405.
5
The EcoRI restriction endonuclease, covalently closed DNA and ethidium bromide.EcoRI 限制性内切酶、共价闭合 DNA 和溴化乙锭。
Biochem J. 1981 Dec 1;199(3):767-77. doi: 10.1042/bj1990767.
6
The effect of divalent cations on the mode of action of DNase I. The initial reaction products produced from covalently closed circular DNA.二价阳离子对脱氧核糖核酸酶I作用模式的影响。由共价闭合环状DNA产生的初始反应产物。
J Biol Chem. 1980 Apr 25;255(8):3726-35.
7
In vitro analysis of Hin-mediated site-specific recombination.Hin介导的位点特异性重组的体外分析。
Cold Spring Harb Symp Quant Biol. 1984;49:751-60. doi: 10.1101/sqb.1984.049.01.085.
8
The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.闭环DNA与嵌入染料的相互作用。I. 存在和不存在染料时SV40 DNA的超螺旋密度。
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Gene Amplif Anal. 1987;5:103-18.
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Site-specific recombination and topoisomerization by Tn21 resolvase: role of metal ions.Tn21 解离酶介导的位点特异性重组和拓扑异构化：金属离子的作用
Nucleic Acids Res. 1986 Sep 25;14(18):7213-26. doi: 10.1093/nar/14.18.7213.