Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA.
作者信息
Pritchard K H, Seifert H S
机构信息
Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, IL 60611, USA.
出版信息
Mol Biotechnol. 1995 Dec;4(3):315-7. doi: 10.1007/BF02779023.
PMID:8680936
Abstract
The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation completely abrogates transformation.
摘要
Zotero 插件