Kazemi R B, Spångberg L S
Department of Restorative Dentistry and Endodontology, University of Connecticut Health Center, Farmington 06030-1715, USA.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1995 Dec;80(6):720-5. doi: 10.1016/s1079-2104(05)80257-4.
This study determined the correlation between the degree of dye penetration in standardized voids to various reduced pressures.
Standardized voids of 0.50 and 0.22 mm in diameter were created in 60 human roots; specimens were divided into five groups. Four groups were subjected to a 2% filtered aqueous methylene blue dye solution under different conditions of atmospheric pressure for 7 days and reduced pressures of a 10 torr, a 260 torr, and a 510 torr for 30 minutes. The conditions for the fifth group were similar to the last group except that ethyl alcohol was substituted for water in the dye.
All positive control specimens showed complete dye penetration. The least linear dye penetration was observed in passively immersed specimens. The results were significantly different from those when reduced pressure had been used (p < 0.001). There were no significant differences among the groups with reduced pressure. No significant difference was found at any pressure level between the means of dye diffusion in the 0.50 and the 0.22 mm voids. The smallest standard deviations were observed for the most reduced air pressure of 10 torr, followed by the group of the least reduced pressure of 510 torr, with the use of the tincture dye solution.
A 10-torr reduced pressure and an application of the tincture dye solution would provide an improved method for microleakage studies.
本研究确定标准化空洞中染料渗透程度与不同减压程度之间的相关性。
在60颗人牙根中制备直径为0.50毫米和0.22毫米的标准化空洞；将标本分为五组。四组在不同大气压条件下，用2%过滤亚甲蓝染料水溶液处理7天，并在10托、260托和510托的减压条件下处理30分钟。第五组的条件与最后一组相似，只是染料中的水被乙醇替代。
所有阳性对照标本均显示染料完全渗透。被动浸泡的标本中观察到的线性染料渗透最少。结果与使用减压时的结果有显著差异（p < 0.001）。减压组之间无显著差异。在任何压力水平下，0.50毫米和0.22毫米空洞中染料扩散平均值之间均未发现显著差异。使用酊剂染料溶液时，在10托的最大减压下观察到最小的标准差，其次是510托的最小减压组。
10托的减压和酊剂染料溶液的应用将为微渗漏研究提供一种改进的方法。
