Navarro Becerra N, Munaro N I
Cátedra de Farmacología; Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa, Argentina.
Neuropharmacology. 1996 Jan;35(1):57-61. doi: 10.1016/0028-3908(95)00120-4.
Changes is glutamate decarboxylase activity (GAD) in the main (MOB) and accessory (AOB) olfactory bulbs were determined at 11:00 hr and 17:00 hr in intact odor deprived (IOD) male rats exposed to female olfactory stimuli, and in castrated odor deprived males (COD) either injected with testosterone (T), exposed to female pheromone or injected with T and exposed to pheromone. Grouping IOD males by female olfactory stimulus and time of the day. MOB and AOB-GAD activity changed in the morning and not in the afternoon. In COD males, T injection induced an increased response in MOB-GAD activity 24 hr later either in the morning or in the afternoon, while no changes were seen at 11:00 or 17:00 hr exposure to female odor. In the AOB of COD males, both T administration or exposure to female pheromone, only induced an increase of enzyme activity in the morning. The association of T and female pheromone, decreased morning GAD activity both in the MOB and in the AOB compared with the values of COD males treated with T. In the afternoon this association had no effect compared with MOB, AOB-GAD activity of COD males injected with testosterone. These results indicate that hormonal and olfactory inputs in IOD and COD males are effective in changing olfactory bulb-GAD activity mainly in the morning.
在上午11点和下午5点，测定了完整的嗅觉剥夺（IOD）雄性大鼠暴露于雌性嗅觉刺激下，以及阉割后的嗅觉剥夺雄性大鼠（COD）注射睾酮（T）、暴露于雌性信息素或注射T并暴露于信息素时，主嗅球（MOB）和副嗅球（AOB）中谷氨酸脱羧酶活性（GAD）的变化。将IOD雄性大鼠按雌性嗅觉刺激和一天中的时间分组。MOB和AOB - GAD活性在上午发生变化，下午未发生变化。在COD雄性大鼠中，注射T在24小时后无论是上午还是下午均会导致MOB - GAD活性增加，而在上午11点或下午5点暴露于雌性气味时未观察到变化。在COD雄性大鼠的AOB中，给予T或暴露于雌性信息素仅在上午诱导酶活性增加。与注射T的COD雄性大鼠相比，T与雌性信息素的联合作用使MOB和AOB中的上午GAD活性均降低。在下午，与注射睾酮的COD雄性大鼠的MOB、AOB - GAD活性相比，这种联合作用没有影响。这些结果表明，IOD和COD雄性大鼠中的激素和嗅觉输入主要在上午有效地改变嗅球 - GAD活性。
