拟南芥质体外油酸去饱和酶基因（FAD2）在酿酒酵母中的功能表达
Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae.
作者信息
Covello P S, Reed D W
机构信息
National Research Council, Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada.
出版信息
Plant Physiol. 1996 May;111(1):223-6. doi: 10.1104/pp.111.1.223.
Abstract
The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces cerevisiae cells and were confirmed to be linoleic acid and delta 9, delta 12-hexadecadienoic acid by gas chromatography-mass spectrometry.
摘要
本文报道了拟南芥FAD2基因（编码质体外油酸去饱和酶，即1-酰基-2-油酰基-sn-甘油-3-磷酸胆碱δ12-去饱和酶）在酵母中的功能表达。在转化的酿酒酵母细胞中，二烯脂肪酸占总脂肪酸的比例高达11%（w/w），通过气相色谱-质谱联用技术证实其为亚油酸和δ9,δ12-十六碳二烯酸。
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