Cíz M, Lojek A, Hosek B, Feilhauerová M
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
Toxicol Lett. 1996 Jul;86(1):39-45. doi: 10.1016/0378-4274(96)03657-0.
The luminol-enhanced chemiluminescence (CL) activity of splenocytes of mice of the strain (CBA x C57B1)F1 was monitored after treatment with Cd2+ (cadmium chloride) in both in vitro and in vivo experiments. Cd2+ (at concentrations of 1 microM-1 mM) increased the CL reaction of the splenocytes (2 x 10(6) cells/ml) in vitro in a dose-dependent manner, both instantaneously and after incubation for 1 h. In in vivo experiments, Cd2+ was administered in two ways. Following a 14-day administration of cadmium to mice in drinking water (300 mg Cd2+/l), the CL reaction of the splenocytes was significantly reduced. On the other hand, after i.p. administration of CdCl2 dissolved in PBS (2 mg/kg body mass, repeated seven times during 14 days), the metabolic activity of the phagocytes was increased. From the results it follows that cadmium affects the immune system. However, its toxicity is dependent on the route of administration.
在体外和体内实验中，在用Cd2 +（氯化镉）处理后，监测（CBA×C57B1）F1品系小鼠脾细胞的鲁米诺增强化学发光（CL）活性。Cd2 +（浓度为1 microM - 1 mM）在体外以剂量依赖的方式增加脾细胞（2×10（6）个细胞/ml）的CL反应，无论是即时还是孵育1小时后。在体内实验中，Cd2 +以两种方式给药。在给小鼠饮用含镉水（300 mg Cd2 + / l）14天后，脾细胞的CL反应显著降低。另一方面，在腹腔注射溶解于PBS中的CdCl2（2 mg / kg体重，在14天内重复7次）后，吞噬细胞的代谢活性增加。从结果可以看出，镉会影响免疫系统。然而，其毒性取决于给药途径。
