Di Biagio E, Sánchez-Borges M, Desenne J J, Suárez-Chacón R, Somoza R, Acquatella G
Immunology Service, Instituto de Oncología y Hematología, Ministry of Health, Caracas, Venezuela.
Int Arch Allergy Immunol. 1996 Jul;110(3):244-51. doi: 10.1159/000237294.
In order to clarify the mechanisms of the eosinophilia frequently observed in patients with Hodgkin's disease (HD), 18 patients and 16 age- and sex-matched controls were studied. Increased eosinophil numbers in peripheral blood and serum IgE, as well as decreased cell-mediated immunity were present in HD patients compared with control individuals. Advanced disease was accompanied by lower eosinophil levels, increased IgE, and lower CD4+ T cell counts in peripheral blood. Eosinophilia correlated with CD4+ T cell counts, suggesting that eosinophil production could be under CD4+ T cell control. GM-CSF production in vitro by Phytohemagglutinin-stimulated mononuclear cells was significantly lower in HD patients with eosinophilia. On the other hand, an eosinophil-survival-enhancing activity was found in sera and culture supernatants from controls and HD patients; this activity was stronger for HD patients and was higher for those with eosinophilia. Furthermore, this activity was completely abolished by preincubation with monoclonal antibodies to IL-5, but not with normal mouse serum. Our results suggest that defects of cell-mediated immunity present in patients with HD are accompanied by a predominant type 2 cytokine profile. IL-5 is involved in the increased eosinophil production observed in these patients.
为了阐明霍奇金淋巴瘤（HD）患者中常见的嗜酸性粒细胞增多的机制，我们对18例患者以及16例年龄和性别匹配的对照者进行了研究。与对照个体相比，HD患者外周血嗜酸性粒细胞数量增加、血清IgE升高，且细胞介导的免疫功能降低。晚期疾病患者外周血嗜酸性粒细胞水平较低、IgE升高以及CD4 + T细胞计数降低。嗜酸性粒细胞增多与CD4 + T细胞计数相关，提示嗜酸性粒细胞的产生可能受CD4 + T细胞控制。嗜酸性粒细胞增多的HD患者中，植物血凝素刺激的单核细胞体外GM-CSF产生显著降低。另一方面，在对照者和HD患者的血清及培养上清液中发现了一种嗜酸性粒细胞存活增强活性；HD患者的这种活性更强，嗜酸性粒细胞增多的患者更高。此外，用抗IL-5单克隆抗体预孵育可完全消除这种活性，而正常小鼠血清则无此作用。我们的结果表明，HD患者存在的细胞介导免疫缺陷伴随着主要的2型细胞因子谱。IL-5参与了这些患者中观察到的嗜酸性粒细胞产生增加。
