Yamamoto H, Yamamoto I
Laboratory of Pathology, Kohno Clinical Medicine Research Institute, Tokyo, Japan.
Exp Anim. 1996 Jan;45(1):45-54. doi: 10.1538/expanim.45.45.
A tumor developed spontaneously in the subcutaneous tissue of the hind leg of a 7-month-old female ddY mouse. Light and electron microscopical examinations revealed that the original tumor was composed of an admixture of fibroblast-like and histiocyte-like cells arranged predominantly in a storiform or cartwheel pattern. The tumor cells gave positive reactions for acid phosphatase, N-acetyl-beta-glucosaminidase, non-specific esterase, beta-glucuronidase, alpha-1 antitrypsin and fibronectin. The original tumor was diagnosed as a malignant fibrous histiocytoma (MFH). The tumor was serially transplanted into syngeneic mice up to the 92nd generation. The tumor was also consistently transplanted into allogeneic mice of several inbred strains. The allogeneic mice used in the present study were strains having different H-2 haplotypes. During succeeding passages, transplanted tumors showed aberrant growth properties. The tumor transplanted into mice of inbred strains took well to back transplantation for mice of original strain and allotransplantation for other inbred strains. The pathological features of these transplantable tumors were basically similar to those of the original tumor. As mentioned above, a MFH developed spontaneously in the ddY mouse was consistently transplantable into both syngeneic and allogeneic mice.
一只7个月大的雌性ddY小鼠后肢皮下组织自发长出一个肿瘤。光镜和电镜检查显示，原发肿瘤由成纤维细胞样和组织细胞样细胞混合组成，主要呈席纹状或车轮状排列。肿瘤细胞对酸性磷酸酶、N-乙酰-β-氨基葡萄糖苷酶、非特异性酯酶、β-葡萄糖醛酸酶、α-1抗胰蛋白酶和纤连蛋白呈阳性反应。原发肿瘤被诊断为恶性纤维组织细胞瘤（MFH）。该肿瘤连续移植到同基因小鼠中直至第92代。该肿瘤也始终能移植到几种近交系的异基因小鼠中。本研究中使用的异基因小鼠是具有不同H-2单倍型的品系。在后续传代过程中，移植瘤表现出异常的生长特性。移植到近交系小鼠中的肿瘤很容易回输给原品系小鼠，并能同种异体移植到其他近交系小鼠中。这些可移植肿瘤的病理特征与原发肿瘤基本相似。如上所述，ddY小鼠自发形成的MFH始终能移植到同基因和异基因小鼠中。
