Salmeron J M, Oldroyd G E, Rommens C M, Scofield S R, Kim H S, Lavelle D T, Dahlbeck D, Staskawicz B J
Department of Plant Biology, University of California, Berkeley 94720, USA.
Cell. 1996 Jul 12;86(1):123-33. doi: 10.1016/s0092-8674(00)80083-5.
In tomato, resistance to Pseudomonas syringae pv. tomato (Pst) strains expressing the avirulence gene avrPto requires the presence of at least two host genes, designated Pto and Prf. Here we report that Prf encodes a protein with leucine-zipper, nucleotide-binding, and leucine-rich repeat motifs, as are found in a number of resistance gene products from other plants. prf mutant alleles (4) were found to carry alterations within the Prf coding sequence. A genomic fragment containing Prf complemented a prf mutant tomato line both for resistance to Pst strains expressing avrPto and for sensitivity to the insecticide Fenthion. Prf resides in the middle of the Pto gene cluster, 24 kb from the Pto gene and 500 bp from the Fen gene.
在番茄中，对表达无毒基因avrPto的丁香假单胞菌番茄致病变种（Pst）菌株的抗性需要至少两个宿主基因的存在，分别命名为Pto和Prf。我们在此报告，Prf编码一种具有亮氨酸拉链、核苷酸结合和富含亮氨酸重复基序的蛋白质，这与其他植物的许多抗性基因产物中发现的基序相同。发现prf突变等位基因（4个）在Prf编码序列内发生了改变。一个包含Prf的基因组片段对一个prf突变番茄品系进行了互补，使其对表达avrPto的Pst菌株具有抗性，并对杀虫剂倍硫磷敏感。Prf位于Pto基因簇的中间，距离Pto基因24 kb，距离Fen基因500 bp。
