Harrison G B, Heath D D, Dempster R P, Gauci C, Newton S E, Cameron W G, Robinson C M, Lawrence S B, Lightowlers M W, Rickard M D
Mallinckrodt Veterinary New Zealand Ltd, Development Department, Upper Hutt, New Zealand.
Int J Parasitol. 1996 Feb;26(2):195-204. doi: 10.1016/0020-7519(95)00097-6.
Oncosphere antigens of Taenia ovis were solubilised in sodium dodecyl sulphate and separated by electrophoresis in polyacrylamide gels (SDS-PAGE). Antigen-containing gel fractions cut from the region covering 18-12 kDa were shown to be highly immunogenic in sheep challenge experiments. Specific antisera against 2 candidate antigens at 18 and 16 kDa were used to screen a cDNA library prepared from T. ovis oncosphere mRNA. Recombinant proteins selected with antibody to the 16 and 18 kDa native antigens were expressed as GST fusion proteins. Vaccination trials using either of the 2 fusion proteins To16.17-GST and To18-GST, revealed that each was capable of inducing high levels of immunity in sheep against challenge infection with T. ovis eggs. Antibodies induced by vaccination with the recombinant antigens reacted specifically with their respective 18 or 16 kDa native oncosphere antigens. There was no apparent homology between the T. ovis cDNA coding for To18 and To16.17, or with another host-protective antigen, To45W, described previously. These additional host-protective antigens should prove a valuable adjunct to To45W and permit the development of effective vaccination strategies.
绵羊带绦虫六钩蚴抗原在十二烷基硫酸钠中溶解，并通过聚丙烯酰胺凝胶电泳（SDS-PAGE）进行分离。在绵羊攻毒实验中，从覆盖18 - 12 kDa区域切下的含抗原凝胶组分显示出高度免疫原性。使用针对18 kDa和16 kDa的2种候选抗原的特异性抗血清筛选从绵羊带绦虫六钩蚴mRNA制备的cDNA文库。用针对16 kDa和18 kDa天然抗原的抗体筛选出的重组蛋白表达为GST融合蛋白。使用2种融合蛋白To16.17-GST和To18-GST中的任何一种进行疫苗接种试验，结果表明每种融合蛋白都能够在绵羊中诱导高水平的免疫力，使其抵抗绵羊带绦虫卵的攻毒感染。用重组抗原接种诱导产生的抗体与它们各自的18 kDa或16 kDa天然六钩蚴抗原发生特异性反应。编码To18和To16.17的绵羊带绦虫cDNA之间，或与先前描述的另一种宿主保护性抗原To45W之间没有明显的同源性。这些额外的宿主保护性抗原应被证明是To45W的有价值辅助物，并有助于开发有效的疫苗接种策略。
