两种病毒易感性检测方法的盲法比较：蚀斑减少试验与微孔板原位酶联免疫吸附测定法。

A blinded comparison of two methods of viral susceptibility testing: plaque reduction assay versus microplate in situ ELISA.

作者信息

Leahy B J, Christiansen K J, Collins P

机构信息

Department of Microbiology, Royal Perth Hospital, Australia.

出版信息

J Virol Methods. 1996 Jan;56(1):85-90. doi: 10.1016/0166-0934(95)01957-x.

DOI:10.1016/0166-0934(95)01957-x

PMID:8690771

Abstract

Viral susceptibility testing has been shown to have a role in the management of patients with herpes simplex infections. In this study, 25 isolates of herpes simplex virus representing a broad spectrum of acyclovir-susceptible and -resistant phenotypes were tested using a microplate in situ enzyme-linked immunosorbent assay (MISE). This method is objective and more rapid than the traditional plaque reduction assay (PRA). The previously derived PRA results were not known at the time of testing with the MISE method. The correlation coefficient between PRA and MISE was 0.85. Agreement on sensitive or resistant was reached for 21 of 25 isolates. The standardised microplate in situ ELISA was found to be an acceptable alternative to the plaque reduction assay.

摘要

病毒敏感性检测已被证明在单纯疱疹感染患者的管理中发挥作用。在本研究中，使用微孔板原位酶联免疫吸附测定（MISE）对25株代表广泛阿昔洛韦敏感和耐药表型的单纯疱疹病毒分离株进行了检测。该方法客观且比传统的蚀斑减少测定（PRA）更快。在用MISE方法检测时，之前得出的PRA结果尚不可知。PRA与MISE之间的相关系数为0.85。25株分离株中有21株在敏感或耐药方面达成了一致。标准化微孔板原位ELISA被发现是蚀斑减少测定的一种可接受的替代方法。