ELVIRA HSV，一种用于快速单纯疱疹病毒药敏试验的产量降低测定法。
ELVIRA HSV, a yield reduction assay for rapid herpes simplex virus susceptibility testing.
作者信息
Stránská Ruzena, Schuurman Rob, Scholl David R, Jollick Joseph A, Shaw Carl J, Loef Caroline, Polman Merjo, van Loon Anton M
机构信息
Department of Virology, Eijkman-Winkler Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
出版信息
Antimicrob Agents Chemother. 2004 Jun;48(6):2331-3. doi: 10.1128/AAC.48.6.2331-2333.2004.
Abstract
A colorimetric yield reduction assay, ELVIRA (enzyme-linked virus inhibitor reporter assay) HSV, was developed to determine the antiviral drug susceptibilities of herpes simplex virus (HSV). It uses an HSV-inducible reporter cell line. This simple and rapid assay has an objective readout, low inoculum size, and good reproducibility. The results correlate well with those of the plaque reduction assay.
摘要
一种比色法产量降低试验，即ELVIRA（酶联病毒抑制剂报告试验）单纯疱疹病毒试验，被开发用于确定单纯疱疹病毒（HSV）的抗病毒药物敏感性。它使用一种HSV诱导的报告细胞系。这种简单快速的试验具有客观的读数、低接种量和良好的重复性。结果与蚀斑减少试验的结果相关性良好。
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