Cotarelo M, Catalán P, Sánchez-Carrillo C, Menasalvas A, Cercenado E, Tenorio A, Bouza E
Servicio de Microbiología Clínica y Enfermedades Infecciosas-VIH, Hospital General Universitario 'Gregorio Marañón', Madrid, Spain.
J Antimicrob Chemother. 1999 Nov;44(5):705-8. doi: 10.1093/jac/44.5.705.
We compare a rapid dilution method for the determination of antiviral susceptibility of herpes simplex virus (HSV) with the plaque reduction assay. A total of 84 HSV clinical isolates were studied by both methods to detect in-vitro resistance to acyclovir and foscarnet. The rapid method showed for the detection of HSV isolates resistant to acyclovir and foscarnet, a sensitivity of 96. 8% and 100% and specificity of 100% and 100%, respectively. This method provides an easy and accurate screening procedure for the susceptibility testing of HSV to antiviral agents.
我们将一种用于测定单纯疱疹病毒（HSV）抗病毒敏感性的快速稀释法与蚀斑减少试验进行了比较。通过这两种方法对总共84株HSV临床分离株进行研究，以检测其对阿昔洛韦和膦甲酸钠的体外耐药性。快速法检测对阿昔洛韦和膦甲酸钠耐药的HSV分离株时，敏感性分别为96.8%和100%，特异性均为100%。该方法为HSV对抗病毒药物的敏感性检测提供了一种简便且准确的筛选程序。
