Tohamy A A, el Ghor A A, Moharram N Z, el-Shazly M M
Zoology Department, Faculty of Science, Cairo University, Giza, Egypt.
Mutat Res. 1996 Aug 8;360(3):155-63. doi: 10.1016/s0165-1161(96)90012-6.
The protective role of soybean feeding against the cytogenetic and histopathologic effects of the nitrosamine precursors sodium nitrate and dibutylamine was evaluated. Treated animals were killed every 3 months, over a period of 15 months, and bone marrow cells were prepared for cytogenetic studies and livers for histopathological observations. Structural chromosomal aberrations and mitotic indices increased after treatment with the nitrosamine precursors for all tested times. Livers were within the normal appearance during the first 6 months. After that a mild, moderate, marked dysplasia with lymphocytic infiltration, fatty vacuolation and liver atrophy was observed. Soybean coadministered with the nitrosamine precursors reduced the number of structural chromosomal aberrations. Mitotic indices decreased at all tested groups but still higher than the control level. A marked reduction in dysplastic features in the liver cells was observed. In conclusion, the cytogenetic and histopathologic results of this study strongly support the protective role of soybean against the genotoxic and carcinogenic action of nitrosomine formed in vivo from its precursors.
评估了大豆喂养对亚硝胺前体硝酸钠和二丁胺的细胞遗传学和组织病理学影响的保护作用。在15个月的时间里，每3个月处死一批接受治疗的动物，制备骨髓细胞用于细胞遗传学研究，制备肝脏用于组织病理学观察。在所有测试时间，用亚硝胺前体处理后，结构染色体畸变和有丝分裂指数均增加。在最初的6个月里，肝脏外观正常。之后，观察到轻度、中度、明显的发育异常，伴有淋巴细胞浸润、脂肪空泡形成和肝脏萎缩。与亚硝胺前体共同给予大豆可减少结构染色体畸变的数量。所有测试组的有丝分裂指数均下降，但仍高于对照水平。观察到肝细胞发育异常特征明显减少。总之，本研究的细胞遗传学和组织病理学结果有力地支持了大豆对体内由其前体形成的亚硝胺的遗传毒性和致癌作用的保护作用。
