Huhtala A, Huikuri K T, Palkama A, Tervo T
Anat Rec. 1977 Jun;188(2):263-71. doi: 10.1002/ar.1091880210.
The innervation of the rat Harderian gland was studied using histochemical methods for catecholamines and acetylcholinesterase (AChE). Selective denervations were performed to investigate the neural connections of this gland with various ganglia. Light microscopically the AChE-positive nerves seemed to run as thick bundles in the intertubular connective tissue. These bundles sent finer branches around the acini. The blood vessels, localized in the connective tissue septa, were surrounded by a dense plexus of AChE-containing fibres. By electron microscopy, the AChE-positive fibres were seen to terminate near the myoepithelial cells surrounding secretory cells. These fibres were also observed in contact with the blood vessels and occasionally close to the secretory cells. Fluorescent adrenergic nerves surrounded the blood vessels. Some fibres were also observed in the interlobular tissue. All the AChE-containing nerves degenerated after cutting the zygomatic nerve. On the other hand, removal of the ciliary ganglion or the superior cervical ganglion, or stereotactic coagulation of the ophthalmic nerve did not affect these nerves. The fluorescent adrenergic fibres disappeared following both removal of the superior cervical ganglion and coagulation of the ophthalmic nerve. These fibres were intact after removal of the ciliary ganglion.
利用儿茶酚胺和乙酰胆碱酯酶（AChE）的组织化学方法研究了大鼠哈德氏腺的神经支配。进行选择性去神经支配以研究该腺体与各种神经节的神经连接。光镜下，AChE阳性神经似乎以粗大束状走行于小管间结缔组织中。这些束在腺泡周围发出更细的分支。位于结缔组织间隔中的血管被含AChE纤维的致密丛包围。电镜下，可见AChE阳性纤维在围绕分泌细胞的肌上皮细胞附近终止。还观察到这些纤维与血管接触，偶尔靠近分泌细胞。荧光肾上腺素能神经围绕血管。在小叶间组织中也观察到一些纤维。切断颧神经后，所有含AChE的神经均发生退变。另一方面，切除睫状神经节或颈上神经节，或对视神经进行立体定向凝固，均不影响这些神经。切除颈上神经节和凝固视神经后，荧光肾上腺素能纤维消失。切除睫状神经节后，这些纤维保持完整。
