Kim U J, Shizuya H, Kang H L, Choi S S, Garrett C L, Smink L J, Birren B W, Korenberg J R, Dunham I, Simon M I
Division of Biology, California Institute of Technology, Pasadena, 91125, USA.
Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6297-301. doi: 10.1073/pnas.93.13.6297.
We have constructed a physical map of human chromosome 22q using bacterial artificial chromosome (BAC) clones. The map consists of 613 chromosome 22-specific BAC clones that have been localized and assembled into contigs using 452 landmarks, 346 of which were previously ordered and mapped to specific regions of the q arm of the chromosome by means of chromosome 22-specific yeast artificial chromosome clones. The BAC-based map provides immediate access to clones that are stable and convenient for direct genome analysis. The approach to rapidly developing marker-specific BAC contigs is relatively straightforward and can be extended to generate scaffold BAC contig maps of the rest of the chromosomes. These contigs will provide substrates for sequencing the entire human genome. We discuss how to efficiently close contig gaps using the end sequences of BAC clone inserts.
我们利用细菌人工染色体（BAC）克隆构建了人类22号染色体q臂的物理图谱。该图谱由613个22号染色体特异性BAC克隆组成，这些克隆已通过452个标记定位并组装成重叠群，其中346个标记先前已借助22号染色体特异性酵母人工染色体克隆进行排序并定位到染色体q臂的特定区域。基于BAC的图谱可直接获取稳定且便于进行直接基因组分析的克隆。快速构建标记特异性BAC重叠群的方法相对简单，并且可以扩展用于生成其余染色体的支架BAC重叠群图谱。这些重叠群将为整个人类基因组测序提供底物。我们讨论了如何利用BAC克隆插入片段的末端序列有效填补重叠群间隙。
