乙型肝炎DNA疫苗：黑猩猩免疫原性的证据及与其他疫苗的比较
DNA vaccine for hepatitis B: evidence for immunogenicity in chimpanzees and comparison with other vaccines.
作者信息
Davis H L, McCluskie M J, Gerin J L, Purcell R H
机构信息
Loeb Medical Research Institute, Ottawa Civic Hospital, ON, Canada.
出版信息
Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7213-8. doi: 10.1073/pnas.93.14.7213.
Abstract
Vaccination of two chimpanzees against hepatitis B virus (HBV) by intramuscular injection of plasmid DNA encoding the major and middle HBV envelope proteins induced group-, subtype- and preS2-specific antibodies. These were initially of IgM isotype, and then they were of IgG (predominantly IgGl) isotype. The chimpanzee injected with 2 mg of DNA attained >100 milli-international units/ml of anti-HBs antibody after one injection and 14,000 milli-international units/ml after four injections. A smaller dose (400 microg) induced lower and transient titers, but a strong anamnestic response occurred 1 year later. Comparison with responses in 23 chimpanzees receiving various antigen-based HBV vaccines suggests that the DNA approach is promising for prophylactic immunization against HBV.
摘要
通过肌肉注射编码乙肝病毒（HBV）主要和中包膜蛋白的质粒DNA对两只黑猩猩进行乙肝疫苗接种，诱导产生了组特异性、亚型特异性和前S2特异性抗体。这些抗体最初为IgM同种型，随后为IgG（主要是IgG1）同种型。注射2毫克DNA的黑猩猩在一次注射后抗-HBs抗体达到>100毫国际单位/毫升，四次注射后达到14000毫国际单位/毫升。较小剂量（400微克）诱导产生较低且短暂的滴度，但1年后出现强烈的回忆反应。与23只接受各种基于抗原的乙肝疫苗的黑猩猩的反应进行比较表明，DNA方法在预防乙肝病毒免疫方面很有前景。
相似文献
1
DNA vaccine for hepatitis B: evidence for immunogenicity in chimpanzees and comparison with other vaccines.乙型肝炎DNA疫苗：黑猩猩免疫原性的证据及与其他疫苗的比较
Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7213-8. doi: 10.1073/pnas.93.14.7213.
2
DNA-based immunization against the envelope proteins of the hepatitis B virus.
J Biotechnol. 1996 Jan 26;44(1-3):47-57. doi: 10.1016/0168-1656(95)00098-4.
3
Testing of CpG-optimized protein and DNA vaccines against the hepatitis B virus in chimpanzees for immunogenicity and protection from challenge.对经CpG优化的蛋白质和DNA疫苗进行黑猩猩乙型肝炎病毒免疫原性测试及攻毒保护试验。
Intervirology. 2006;49(3):144-51. doi: 10.1159/000089375.
4
Neutralization of hepatitis B virus (HBV) by human monoclonal antibody against HBV surface antigen (HBsAg) in chimpanzees.在黑猩猩中，人源抗乙肝病毒表面抗原（HBsAg）单克隆抗体对乙肝病毒（HBV）的中和作用。
Antiviral Res. 2008 Sep;79(3):188-91. doi: 10.1016/j.antiviral.2008.03.006. Epub 2008 Apr 28.
5
Successful postexposure vaccination against hepatitis B in chimpanzees.
J Med Virol. 1988 Aug;25(4):433-9. doi: 10.1002/jmv.1890250407.
6
Immunogenicity in chimpanzees of experimental hepatitis B vaccines prepared from intact hepatitis B virus, purified polypeptides, or polypeptide micelles.由完整乙肝病毒、纯化多肽或多肽微胶粒制备的实验性乙肝疫苗在黑猩猩体内的免疫原性。
J Med Virol. 1982;10(1):65-74. doi: 10.1002/jmv.1890100109.
7
Licensed recombinant hepatitis B vaccines protect chimpanzees against infection with the prototype surface gene mutant of hepatitis B virus.获得许可的重组乙型肝炎疫苗可保护黑猩猩免受乙型肝炎病毒原型表面基因突变体的感染。
Hepatology. 1999 Sep;30(3):779-86. doi: 10.1002/hep.510300309.
8
Cloning, protein expression and immunogenicity of HBs-murine IL-18 fusion DNA vaccine.乙肝表面抗原-小鼠白细胞介素-18融合DNA疫苗的克隆、蛋白表达及免疫原性
Asian Pac J Allergy Immunol. 2007 Dec;25(4):233-42.
9
Protective effect of a synthetic peptide comprising the complete preS2 region of the hepatitis B virus surface protein.包含乙肝病毒表面蛋白完整前S2区的合成肽的保护作用
J Med Virol. 1989 May;28(1):7-12. doi: 10.1002/jmv.1890280103.
10
DNA-mediated immunization to the hepatitis B surface antigen in mice: aspects of the humoral response mimic hepatitis B viral infection in humans.小鼠中针对乙型肝炎表面抗原的DNA介导免疫：体液免疫反应的某些方面模拟人类的乙型肝炎病毒感染。
Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5307-11. doi: 10.1073/pnas.92.12.5307.
引用本文的文献
1
Novel mechanism of gene transfection by low-energy shock wave.低能量冲击波介导基因转染的新机制
Sci Rep. 2015 Aug 5;5:12843. doi: 10.1038/srep12843.
2
Induced immunity against hepatitis B virus.诱导的抗乙型肝炎病毒免疫力。
World J Hepatol. 2015 Jun 28;7(12):1660-70. doi: 10.4254/wjh.v7.i12.1660.
3
The chimpanzee model for hepatitis B virus infection.乙型肝炎病毒感染的黑猩猩模型。
Cold Spring Harb Perspect Med. 2015 Jun 1;5(6):a021469. doi: 10.1101/cshperspect.a021469.
4
Cationic lipid-formulated DNA vaccine against hepatitis B virus: immunogenicity of MIDGE-Th1 vectors encoding small and large surface antigen in comparison to a licensed protein vaccine.阳离子脂质体配方的乙型肝炎病毒DNA疫苗：与市售蛋白疫苗相比，编码小表面抗原和大表面抗原的MIDGE-Th1载体的免疫原性
PLoS One. 2014 Jul 3;9(7):e101715. doi: 10.1371/journal.pone.0101715. eCollection 2014.
5
Cimetidine synergizes with Praziquantel to enhance the immune response of HBV DNA vaccine via activating cytotoxic CD8(+) T cell.西咪替丁与吡喹酮协同作用，通过激活细胞毒性CD8(+) T细胞增强乙肝病毒DNA疫苗的免疫反应。
Hum Vaccin Immunother. 2014;10(6):1688-99. doi: 10.4161/hv.28517. Epub 2014 Mar 18.
6
Preclinical study on combined chemo- and nonviral gene therapy for sensitization of melanoma using a human TNF-alpha expressing MIDGE DNA vector.使用表达人肿瘤坏死因子-α的MIDGE DNA载体进行联合化学疗法和非病毒基因疗法使黑色素瘤致敏的临床前研究。
Mol Oncol. 2014 May;8(3):609-19. doi: 10.1016/j.molonc.2013.12.019. Epub 2014 Jan 18.
7
Enhanced effect of DNA immunization plus in vivo electroporation with a combination of hepatitis B virus core-PreS1 and S-PreS1 plasmids.乙肝病毒核心-PreS1和S-PreS1质粒联合DNA免疫加体内电穿孔的增强效果。
Clin Vaccine Immunol. 2011 Nov;18(11):1789-95. doi: 10.1128/CVI.05113-11. Epub 2011 Sep 7.
8
Construction and characterization of calreticulin-HBsAg fusion gene recombinant adenovirus expression vector.构建并鉴定钙网织蛋白-HBsAg 融合基因重组腺病毒表达载体。
World J Gastroenterol. 2010 Jun 28;16(24):3078-82. doi: 10.3748/wjg.v16.i24.3078.
9
Changes to the natural killer cell repertoire after therapeutic hepatitis B DNA vaccination.治疗性乙型肝炎 DNA 疫苗接种后自然杀伤细胞库的变化。
PLoS One. 2010 Jan 18;5(1):e8761. doi: 10.1371/journal.pone.0008761.
10
Protective anti-hepatitis B virus responses in rhesus monkeys primed with a vectored measles virus and boosted with a single dose of hepatitis B surface antigen.用载体麻疹病毒初免并用单剂量乙型肝炎表面抗原加强免疫的恒河猴体内的保护性抗乙型肝炎病毒反应
J Virol. 2009 Sep;83(17):9013-7. doi: 10.1128/JVI.00906-09. Epub 2009 Jun 17.
本文引用的文献
1
Having a blast with gene transfer.
Curr Biol. 1992 Jan;2(1):30-2. doi: 10.1016/0960-9822(92)90421-6.
2
Direct gene transfer into skeletal muscle in vivo: factors affecting efficiency of transfer and stability of expression.体内将基因直接导入骨骼肌：影响转移效率和表达稳定性的因素。
Hum Gene Ther. 1993 Apr;4(2):151-9. doi: 10.1089/hum.1993.4.2-151.
3
DNA-based immunization induces continuous secretion of hepatitis B surface antigen and high levels of circulating antibody.基于DNA的免疫接种可诱导乙肝表面抗原持续分泌及高水平循环抗体。
Hum Mol Genet. 1993 Nov;2(11):1847-51. doi: 10.1093/hmg/2.11.1847.
4
Intradermal gene immunization: the possible role of DNA uptake in the induction of cellular immunity to viruses.皮内基因免疫：DNA摄取在诱导针对病毒的细胞免疫中的可能作用。
Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9519-23. doi: 10.1073/pnas.91.20.9519.
5
Induction of immunoglobulin synthesis by CD4+ T cell clones.
Semin Immunol. 1993 Dec;5(6):421-30. doi: 10.1006/smim.1993.1048.
6
Direct gene transfer in skeletal muscle: plasmid DNA-based immunization against the hepatitis B virus surface antigen.
Vaccine. 1994 Dec;12(16):1503-9. doi: 10.1016/0264-410x(94)90073-6.
7
DNA-mediated immunization to the hepatitis B surface antigen in mice: aspects of the humoral response mimic hepatitis B viral infection in humans.小鼠中针对乙型肝炎表面抗原的DNA介导免疫：体液免疫反应的某些方面模拟人类的乙型肝炎病毒感染。
Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5307-11. doi: 10.1073/pnas.92.12.5307.
8
Nucleic acid vaccines.核酸疫苗
Clin Microbiol Rev. 1995 Jul;8(3):406-10. doi: 10.1128/CMR.8.3.406.
9
Synthesis and assembly of hepatitis B virus surface antigen particles in yeast.乙型肝炎病毒表面抗原颗粒在酵母中的合成与组装。
Nature. 1982 Jul 22;298(5872):347-50. doi: 10.1038/298347a0.
10
Live recombinant vaccinia virus protects chimpanzees against hepatitis B.活重组痘苗病毒可保护黑猩猩免受乙型肝炎感染。
Nature. 1984;311(5981):67-9. doi: 10.1038/311067a0.
Zotero 插件