Burnside J M, Groot Obbink D J
Department of Microbiology, Royal North Shore Hospital, Australia.
Plasmid. 1996 Jan;35(1):67-70. doi: 10.1006/plas.1996.0008.
Southern hybridization analysis of the IncC plasmid pDGO100 showed that, in addition to the well-characterized integron In7, there is a second integron which is located on a 3.6-kb BamHI fragment. This integron also possesses the qacE delta l and sulI genes typically found as part of the 3'-conserved segment of integrons. The 3.6-kb BamHI fragment, when cloned into pUC19 to form pDGO301, conferred resistance to trimethoprim as well as sulfamethoxazole. The DNA sequence of the trimethoprim resistance gene in pDGO301 was determined and it was shown that the gene was precisely inserted as a cassette in an integron with 100% identity to the trimethoprim resistance gene dfrA7.
对IncC质粒pDGO100的Southern杂交分析表明，除了特征明确的整合子In7外，还有一个位于3.6 kb BamHI片段上的第二个整合子。这个整合子也拥有通常作为整合子3'保守区段一部分而发现的qacE delta l和sulI基因。将3.6 kb BamHI片段克隆到pUC19中形成pDGO301时，赋予了对甲氧苄啶以及磺胺甲恶唑的抗性。测定了pDGO301中甲氧苄啶抗性基因的DNA序列，结果表明该基因作为一个盒式结构精确插入到一个整合子中，与甲氧苄啶抗性基因dfrA7具有100%的同一性。
