西门塔尔牛七个微卫星基因座的评估

Evaluation of seven microsatellite loci in Simmental cattle.

作者信息

Hussein A A, Schmoll F, Führer F, Brem G, Schellander K

机构信息

Institute of Animal Breeding and Genetics, Veterinary University of Vienna, Austria.

出版信息

Zentralbl Veterinarmed A. 1996 Mar;43(1):1-8. doi: 10.1111/j.1439-0442.1996.tb00423.x.

DOI:10.1111/j.1439-0442.1996.tb00423.x

PMID:8693829

Abstract

Seven microsatellite loci were evaluated for their suitability for parentage control. The polymerase chain reaction (PCR) was used to amplify the short tandem repeat (STR) loci in separate reactions. The microsatellite polymorphisms were visualized by radioisotopic autoradiographic detection. The microsatellite loci showed extensive polymorphism with allele numbers ranging from 4-23 and polymorphism information content (PIC) values in the range of 0.57-0.87. The analysis of these loci also revealed that they have a 99.9% combined probability of exclusion (PE) of erroneous parentage. The results of this study revealed that a very high probability of exclusion could be reached with only four microsatellite loci.

摘要

评估了七个微卫星基因座用于亲权鉴定的适用性。采用聚合酶链反应（PCR）在单独反应中扩增短串联重复（STR）基因座。通过放射性同位素放射自显影检测来观察微卫星多态性。这些微卫星基因座显示出广泛的多态性，等位基因数范围为4 - 23，多态性信息含量（PIC）值在0.57 - 0.87之间。对这些基因座的分析还表明，它们对错误亲权的联合排除概率（PE）为99.9%。本研究结果表明，仅使用四个微卫星基因座就能达到非常高的排除概率。