Bjurström S, Carlsten J, Jönsson L
Department of Pathology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Zentralbl Veterinarmed A. 1996 May;43(3):129-38. doi: 10.1111/j.1439-0442.1996.tb00437.x.
Stress-susceptible (SS) pigs develop rhabdomyolysis and increased serum levels of muscle enzymes after a 12 min experimental stress induced by the depolarizing myorelaxant succinylcholine. It is suspected that not only the stress situation but also succinylcholine itself contributes to the skeletal muscle lesions. This experiment was performed to study whether rhabdomyolysis occurs after restraint stress when succinylcholine was replaced by the non-depolarizing myorelaxant pancuronium. Four normal and four SS pigs were subjected to restraint stress by intravenous injection of pancuronium. The neuromuscular block was reversed after 12 min by neostigmine. The animals wee necropsied approximately 48 h after stress and 24 skeletal muscle groups were examined pathologically. The severity of acute myofibre lesions were graded, and the results were compared with the results from normal and SS pigs which had been subjected to restraint stress induced by succinylcholine. The serum levels of creatine kinase (CK) and aspartate aminotransferase (ASAT) stayed at the base line level after the stress. The scores for muscle lesions were significantly lower, both in normal and SS pigs, than after restraint stress induced by succinylcholine indicating no rhabdomyolysis after restraint stress induced by pancuronium. Thus succinylcholine is synergistic with stress, exacerbating its effect on skeletal muscle in SS pigs.
应激敏感（SS）猪在由去极化肌松剂琥珀酰胆碱诱导的12分钟实验性应激后会发生横纹肌溶解，血清肌肉酶水平升高。据推测，不仅应激状态，而且琥珀酰胆碱本身也会导致骨骼肌损伤。进行该实验是为了研究当琥珀酰胆碱被非去极化肌松剂泮库溴铵替代时，束缚应激后是否会发生横纹肌溶解。对4只正常猪和4只SS猪静脉注射泮库溴铵进行束缚应激。12分钟后用新斯的明逆转神经肌肉阻滞。在应激后约48小时对动物进行尸检，并对24个骨骼肌组进行病理检查。对急性肌纤维损伤的严重程度进行分级，并将结果与接受琥珀酰胆碱诱导的束缚应激的正常猪和SS猪的结果进行比较。应激后肌酸激酶（CK）和天冬氨酸转氨酶（ASAT）的血清水平保持在基线水平。正常猪和SS猪的肌肉损伤评分均显著低于琥珀酰胆碱诱导的束缚应激后的评分，表明泮库溴铵诱导的束缚应激后未发生横纹肌溶解。因此，琥珀酰胆碱与应激具有协同作用，会加剧其对SS猪骨骼肌的影响。
