Yazdi H M, Bertrand M A, Wong P T
Department of Laboratory Medicine, Ottawa Civic Hospital, Ontario, Canada.
Acta Cytol. 1996 Jul-Aug;40(4):664-8. doi: 10.1159/000333936.
To study cervical exfoliated cells with Fourier transform infrared spectroscopy (FTIR).
Consecutive samples from 133 women attending the Dysplasia Clinic, Ottawa Civic Hospital, were collected in balanced electrolyte solution. After centrifugation, two smears were prepared for routine screening. The remainder of the pellet was frozen for FTIR spectroscopic study.
In 120 samples, adequate material was available for spectroscopic study. All smears from 17 women with normal spectra were within normal limits (WNL). One hundred three spectra were abnormal. The corresponding smears were interpreted as: 41 low grade squamous intraepithelial lesions, 20 high grade squamous intraepithelial lesions, 6 atypical squamous cells of undetermined significance, 17 cases with benign cellular changes, and 19 WNL. Ten of 17 cases with benign cellular changes had characteristic spectra consistent with inflammatory changes.
FTIR spectroscopy is a highly sensitive technique for detecting cervical abnormalities and a potential tool for prescreening preinvasive lesions of the cervix.
采用傅里叶变换红外光谱（FTIR）对宫颈脱落细胞进行研究。
从渥太华市民医院发育异常门诊就诊的133名女性中连续采集样本，置于平衡电解质溶液中。离心后，制备两张涂片用于常规筛查。剩余的沉淀样本冷冻保存用于FTIR光谱研究。
120份样本有足够的材料用于光谱研究。17名光谱正常女性的所有涂片均在正常范围内（WNL）。103份光谱异常。相应涂片的诊断结果为：41例低级别鳞状上皮内病变，20例高级别鳞状上皮内病变，6例意义不明确的非典型鳞状细胞，17例良性细胞改变，19例WNL。17例良性细胞改变病例中有10例具有与炎症改变一致的特征光谱。
FTIR光谱是检测宫颈异常的高灵敏度技术，也是宫颈浸润前病变预筛查的潜在工具。
