Department of Surgical Oncology, First Hospital of Xi'an Jiaotong University, 710061, Xi'an, China.
Dig Dis Sci. 2010 Sep;55(9):2670-5. doi: 10.1007/s10620-009-1045-4. Epub 2009 Dec 3.
Fourier transform infrared (FTIR) spectroscopy is sensitive to the molecular composition of tissue and has the potential to identify premalignant tissue. Our previous studies found that the lipids band of FTIR decreased in malignant tissues compared to normal tissue but increased in the cell line.
To investigate the change of lipids during carcinogenesis in the gallbladder by FTIR spectroscopy.
The tissue from 12 malignant samples and 10 normal samples together with their corresponding tissue plasma membrane and gallbladder cancer cell lines were observed by FTIR.
Specific changes of lipids were observed in the FTIR spectral features of tissue, cell, and plasma membrane. The CH3 stretching band at 2,957 cm(-1) and the CH2 stretching bands at 2,853 cm(-1) decreased in the malignant tissue but increased in the tissue plasma membrane; the C-O stretching band at 1,740 cm(-1) disappeared in the malignant tissue but significantly increased in the tissue plasma membrane. The intensity of these bands all increased in the cancer cell line. The ratio of intensity (I) of 1,460 cm(-1)/1,398 cm(-1) was smaller in malignant tissue and the tissue plasma membrane.
Lipids were increased in the plasma membrane during carcinogenesis of the gallbladder; the ratio of intensity (I) 1,460 cm(-1)/1,398 cm(-1) could be a marker to diagnose cancer by FTIR.
傅里叶变换红外（FTIR）光谱对组织的分子组成敏感，具有识别癌前组织的潜力。我们之前的研究发现，与正常组织相比，恶性组织的 FTIR 脂质带减少，但在细胞系中增加。
通过 FTIR 光谱研究胆囊癌变过程中脂质的变化。
用 FTIR 观察了 12 个恶性样本和 10 个正常样本的组织及其相应的组织质膜和胆囊癌细胞系。
在组织、细胞和质膜的 FTIR 光谱特征中观察到了脂质的特定变化。2,957 cm(-1)处的 CH3 伸缩带和 2,853 cm(-1)处的 CH2 伸缩带在恶性组织中减少，但在质膜中增加；1,740 cm(-1)处的 C-O 伸缩带在恶性组织中消失，但在质膜中显著增加。这些带的强度在癌细胞系中均增加。1,460 cm(-1)/1,398 cm(-1)处的强度比（I）在恶性组织和质膜中较小。
在胆囊癌变过程中，质膜中的脂质增加；FTIR 诊断癌症的强度比（I）1,460 cm(-1)/1,398 cm(-1)可能是一个标志物。
