AgNOR计数在鉴别浆液性积液中恶性细胞与反应性间皮细胞中的意义。
Significance of AgNOR count in differentiating malignant cells from reactive mesothelial cells in serous effusions.
作者信息
Sujathan K, Kannan S, Pillai K R, Chandralekha B, Amma N S, Nair M K
机构信息
Division of Cytopathology, Regional Cancer Centre, Thiruvananthapuram, Kerala, India.
出版信息
Acta Cytol. 1996 Jul-Aug;40(4):724-8. doi: 10.1159/000333946.
OBJECTIVE
To distinguish reactive mesothelial cells from malignant cells in serous effusions using silver staining of nucleolar organizer regions (AgNOR) applied to ethanol-fixed cytologic preparations.
STUDY DESIGN
One hundred aspirated samples of benign and malignant effusions were studied using the one-step silver staining method. Eight cytologically atypical samples were also included in the study.
RESULTS
In malignant cells the mean AgNOR count was 4.72 +/- 0.76 (+/- SD), and the AgNORs were irregular in shape, while in benign mesothelial cells AgNORs were comparatively larger, single dots with a mean count of 1.92 +/- 0.23. Of the cytologically atypical samples, five were in the malignant range. The others were within benign limits. Repeat cytology of the second aspirate confirmed that finding.
CONCLUSION
AgNOR study appears to be clinically useful as an additional diagnostic tool for use in ascitic and pleural fluid samples when the cytologic diagnosis is difficult.
目的
应用核仁组成区嗜银蛋白（AgNOR）染色法对乙醇固定的细胞学标本进行染色，以鉴别浆液性积液中的反应性间皮细胞和恶性细胞。
研究设计
采用一步嗜银染色法对100份良性和恶性积液的抽吸样本进行研究。研究还纳入了8份细胞学非典型样本。
结果
恶性细胞中AgNOR的平均计数为4.72±0.76（±标准差），且AgNOR形状不规则，而良性间皮细胞中的AgNOR相对较大，呈单个点状，平均计数为1.92±0.23。在细胞学非典型样本中，5份处于恶性范围内。其他样本在良性范围内。对第二次抽吸物进行的重复细胞学检查证实了这一结果。
结论
当细胞学诊断困难时，AgNOR研究作为一种额外的诊断工具，在腹水和胸水样本中似乎具有临床应用价值。
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