Giese M J, Adamu S A, Pitchekian-Halabi H, Ravindranath R M, Mondino B J
Jules Stein Eye Institute, UCLA School of Medicine 90095-1771, USA.
Am J Ophthalmol. 1996 Aug;122(2):245-54. doi: 10.1016/s0002-9394(14)72016-1.
To evaluate the effect of Staphylococcus aureus phage lysate (SPL) vaccination on the development of blepharitis, corneal phlyctenules, and catarrhal infiltrates and on the development of antibodies and the delayed-type hypersensitivity response to S. aureus.
Eighty rabbits received an intradermal immunization of cell wall-complete Freund's adjuvant followed by a booster immunization. Rabbits were given topical applications of viable S. aureus in both eyes and 40 rabbits received subcutaneous SPL vaccinations. Clinical observations were made weekly. An enzyme-linked immunosorbent assay was used to measure IgG, IgA, and IgM antibody levels to ribitol teichoic acid in sera, corneas, and tears. The delayed-type hypersensitivity response was evaluated by skin testing after subcutaneous injection of staphylococcal antigens.
In the SPL-vaccinated group, phlyctenules developed in eight of 40 rabbits while blepharitis developed in 13 of 40. In the nonvaccinated group, phlyctenules developed in three of 40 rabbits and blepharitis developed in five of 40. The number of rabbits with blepharitis was significantly higher in the SPL-vaccinated group than in the nonvaccinated group. In general, the antibody response to ribitol teichoic acid was enhanced, while the delayed-type hypersensitivity response to S. aureus was depressed.
Vaccination with SPL was not found to have a beneficial effect on the development of blepharitis, phlyctenules, and catarrhal infiltrates in our rabbit model.
评估金黄色葡萄球菌噬菌体裂解物（SPL）疫苗接种对睑缘炎、角膜小泡和卡他性浸润的发生发展以及对金黄色葡萄球菌抗体产生和迟发型超敏反应的影响。
80只兔子接受皮内注射细胞壁完全弗氏佐剂，随后进行加强免疫。给兔子双眼局部应用活的金黄色葡萄球菌，40只兔子接受皮下SPL疫苗接种。每周进行临床观察。采用酶联免疫吸附测定法测量血清、角膜和泪液中针对核糖醇磷壁酸的IgG、IgA和IgM抗体水平。通过皮下注射葡萄球菌抗原后进行皮肤试验评估迟发型超敏反应。
在接种SPL疫苗的组中，40只兔子中有8只出现角膜小泡，40只中有13只出现睑缘炎。在未接种疫苗的组中，40只兔子中有3只出现角膜小泡，40只中有5只出现睑缘炎。接种SPL疫苗组中患睑缘炎的兔子数量显著高于未接种疫苗组。总体而言，对核糖醇磷壁酸的抗体反应增强，而对金黄色葡萄球菌的迟发型超敏反应受到抑制。
在我们的兔子模型中，未发现接种SPL疫苗对睑缘炎、角膜小泡和卡他性浸润的发生发展有有益影响。
