Aboagye E O, Bhujwalla Z M
The Johns Hopkins University School of Medicine, Department of Radiology, Baltimore, Maryland 21205, USA.
Cancer Res. 1999 Jan 1;59(1):80-4.
Transduction of mitogenic signals in cells can be mediated by molecules derived from the synthesis and breakdown of the major membrane phospholipid, phosphotidylcholine. Studies were performed on human mammary epithelial cells in culture to understand the impact of malignant transformation and progression on membrane phospholipid metabolism. In the model system used here, phosphocholine levels and total choline-containing phospholipid metabolite levels increased with progression from normal to immortalized to oncogene-transformed to tumor-derived cells. These changes occurred independently of cell doubling time. A "glycerophosphocholine to phosphocholine switch" was apparent with immortalization. This alteration in phenotype of increased phosphocholine relative to glycerophosphocholine was observed in oncogene-transformed and for all human breast tumor cell lines analyzed. The results demonstrate that progression of human mammary epithelial cells from normal to malignant phenotype is associated with altered membrane choline phospholipid metabolism.
细胞中有丝分裂信号的转导可由主要膜磷脂磷脂酰胆碱的合成与分解所衍生的分子介导。对培养中的人乳腺上皮细胞进行了研究，以了解恶性转化和进展对膜磷脂代谢的影响。在此使用的模型系统中，随着细胞从正常状态发展为永生化、癌基因转化再到肿瘤衍生细胞，磷酸胆碱水平和总含胆碱磷脂代谢物水平均有所增加。这些变化与细胞倍增时间无关。永生化时出现了“甘油磷酸胆碱向磷酸胆碱的转变”。在癌基因转化的细胞以及所有分析的人乳腺肿瘤细胞系中，均观察到相对于甘油磷酸胆碱而言磷酸胆碱增加的这种表型改变。结果表明，人乳腺上皮细胞从正常表型向恶性表型的进展与膜胆碱磷脂代谢的改变有关。
