Rodrigo Tapia J P, Martínez Sánchez J A, Sánchez Lazo P, Ramos S, Sampedro Nuño A, Suárez Nieto C
Servicio de ORL, Hospital Central de Asturias.
Acta Otorrinolaringol Esp. 1996 Mar-Apr;47(2):97-103.
Tumoral DNA content was studied by flow cytometry and PCR amplification of c-erbB1 in tissue samples from 31 patients with squamous cell carcinoma of the head and neck. Eighteen cases (58%) were aneuploid and 13 (42%) were diploid. Aneuploidy correlated with pharyngeal site and poorly differentiated tumors, but not with clinical stage or metastases. Six (19.3%) cases had c-erbB1 amplification, which correlated with tumor size, nodal metastasis, poor differentiation, and hypopharyngeal site. Only 20% of patients with amplification survived 30 months, compared with 64% of patients without amplification. None of the patients with aneuploidy and c-erbB1 amplification survived more than 15 months. To conclude, the measurement of cellular DNA content and c-erbB1 amplification seem to have prognostic value in squamous cell carcinoma of the head and neck.
通过流式细胞术和聚合酶链反应（PCR）扩增，对31例头颈部鳞状细胞癌患者组织样本中的肿瘤DNA含量及c-erbB1进行了研究。18例（58%）为非整倍体，13例（42%）为二倍体。非整倍体与咽部位及低分化肿瘤相关，但与临床分期或转移无关。6例（19.3%）出现c-erbB1扩增，其与肿瘤大小、淋巴结转移、低分化及下咽部位相关。扩增患者中仅20%存活超过30个月，而未扩增患者为64%。非整倍体且c-erbB1扩增的患者无一存活超过15个月。总之，细胞DNA含量及c-erbB1扩增的检测对头颈部鳞状细胞癌似乎具有预后价值。
