Curtis M D, Oliver R P
School of Biological Sciences, University of East Anglia, Norwich, UK.
Microbiol Res. 1996 May;151(2):113-9. doi: 10.1016/S0944-5013(96)80034-6.
Gypsy retrotransposons are a major branch of retroid elements and have been found in a wide diversity of eukaryotic organisms including CfT-1 in the tomato leaf mould pathogen, the fungus Cladosporium fulvum (syn. Fulvia fulva). We have examined organisms that are either ecologically or phylogenetically related to C. fulvum, for elements related to CfT-1. Using PCR and Southern hybridisation, similar sequences were found only in the co-genic fungus Cladosporium cladosporioides. This finding confirms the apparent ubiquity of retroelements, suggests that the phylogeny of retrotransposons is consistent with the phylogeny of their hosts and argues against frequent horizontal gene transfer.
吉普赛逆转座子是逆转录元件的一个主要分支，已在多种真核生物中被发现，包括番茄叶霉病原菌（即真菌fulvum（同义词：fulvia fulva））中的CfT-1。我们已经研究了在生态或系统发育上与fulvum相关的生物，寻找与CfT-1相关的元件。使用聚合酶链式反应（PCR）和Southern杂交技术，仅在同基因真菌枝孢霉中发现了相似序列。这一发现证实了逆转录元件明显的普遍性，表明逆转座子的系统发育与其宿主的系统发育一致，并反对频繁的水平基因转移。
