McHale M T, Roberts I N, Noble S M, Beaumont C, Whitehead M P, Seth D, Oliver R P
Norwich Molecular Plant Pathology Group, University of East Anglia, School of Biological Sciences, UK.
Mol Gen Genet. 1992 Jun;233(3):337-47. doi: 10.1007/BF00265429.
A retrotransposon from the fungal tomato pathogen Cladosporium fulvum (syn. Fulvia fulva) has been isolated and characterised. It is 6968 bp in length and bounded by identical long terminal repeats of 427 bp; 5 bp target-site duplications were found. Putative first- and second-strand primer binding sites were identified. Three long open reading frames (ORFs) are predicted from the sequence. The first has homology to retroviral gag genes. The second includes sequences homologous to protease, reverse transcriptase, RNAse H and integrase, in that order. Sequence comparisons of the predicted ORFs indicate that this element is closely related to the gypsy class of LTR retrotransposons. Races of the pathogen exhibit polymorphisms in their complement of at least 25 copies of the sequence. Virus-like particles which co-sediment with reverse transcriptase activity were observed in homogenates of the fungus. This is the first report of an LTR retrotransposon in a filamentous fungus.
从番茄真菌病原体 fulvum（同义词：Fulvia fulva）中分离并鉴定了一种反转录转座子。它长度为6968 bp，由427 bp的相同长末端重复序列界定；发现了5 bp的靶位点重复序列。确定了推定的第一链和第二链引物结合位点。从该序列预测出三个长开放阅读框（ORF）。第一个与逆转录病毒gag基因具有同源性。第二个依次包括与蛋白酶、逆转录酶、RNA酶H和整合酶同源的序列。预测的ORF的序列比较表明，该元件与LTR反转录转座子的gypsy类密切相关。该病原体的不同小种在其至少25个拷贝的序列互补上表现出多态性。在真菌匀浆中观察到与逆转录酶活性共沉降的病毒样颗粒。这是丝状真菌中LTR反转录转座子的首次报道。
