Gilliland R, Williamson K E, Wilson R H, Anderson N H, Hamilton P W
Department of Surgery, Queen's University of Belfast, UK.
Br J Surg. 1996 Jun;83(6):739-49. doi: 10.1002/bjs.1800830607.
The assessment of cell proliferation in colorectal tissue may provide information with both prognostic and therapeutic implications. A variety of methods are available, including flow cytometric estimations of S phase fraction, immunohistochemical and autoradiographic visualization of exogenous and endogenous proliferation proteins, and morphological and stathmokinetic techniques. There is some correlation between Dukes stage and proliferation state features, and there is increased proliferative activity throughout the adenoma-carcinoma sequence. Data on cell proliferation rates are difficult to obtain. When correctly applied, the metaphase arrest technique remains the 'gold standard' of measuring proliferation, but its usefulness in clinical practice is limited. Recent studies have employed dual measurement flow cytometry and double labelling techniques to produce rate data.
结直肠组织中细胞增殖的评估可能会提供具有预后和治疗意义的信息。有多种方法可供使用，包括通过流式细胞术估计S期分数、对外源性和内源性增殖蛋白进行免疫组织化学和放射自显影可视化，以及形态学和静止动力学技术。 Dukes分期与增殖状态特征之间存在一定相关性，并且在腺瘤-癌序列中增殖活性增加。细胞增殖率的数据很难获得。正确应用时，中期阻滞技术仍然是测量增殖的“金标准”，但其在临床实践中的实用性有限。最近的研究采用了双测量流式细胞术和双标记技术来生成速率数据。
