Kubben F J, Peeters-Haesevoets A, Engels L G, Baeten C G, Schutte B, Arends J W, Stockbrügger R W, Blijham G H
Division of Haemato-Oncology, Academic Hospital, Maastricht, The Netherlands.
Gut. 1994 Apr;35(4):530-5. doi: 10.1136/gut.35.4.530.
Immunohistochemistry of the S phase related proliferating cell nuclear antigen (PCNA) was studied as an alternative to ex-vivo bromodeoxyuridine (BrdU) immunohistochemistry for assessment of human colonic cell proliferation. From 16 subjects without colonic disease biopsy specimens were collected from five different sites along the colorectum and processed for BrdU and PCNA immunohistochemistry. The mean proliferation index of PCNA was significantly higher at 133% of the value obtained with BrdU. There was, however, a good correlation between the results from both techniques (r = 0.6275; p < 0.05). Decrease in proliferation index along the colorectum was seen with both staining methods but was clearer with PCNA immunohistochemistry (caecum/ascending colon v rectum: 12.0 v 7.2; p < 0.004). The total number of crypt cells also decreased from proximal to distal (134 to 128; p < 0.06) but at no site correlated significantly with the proliferation index. It is concluded that in clinical cell kinetic studies staining for PCNA may serve as an attractive alternative to the BrdU incorporation assay.
作为评估人类结肠细胞增殖的一种方法，对与S期相关的增殖细胞核抗原（PCNA）进行免疫组织化学研究，以替代体外溴脱氧尿苷（BrdU）免疫组织化学。从16名无结肠疾病的受试者中，沿着结直肠的五个不同部位采集活检标本，并进行BrdU和PCNA免疫组织化学处理。PCNA的平均增殖指数显著高于BrdU所获值的133%。然而，两种技术的结果之间存在良好的相关性（r = 0.6275；p < 0.05）。两种染色方法均可见沿着结直肠增殖指数降低，但PCNA免疫组织化学显示得更明显（盲肠/升结肠与直肠：12.0对7.2；p < 0.004）。隐窝细胞总数也从近端到远端减少（134至128；p < 0.06），但在任何部位均与增殖指数无显著相关性。得出的结论是，在临床细胞动力学研究中，PCNA染色可作为BrdU掺入试验的一种有吸引力的替代方法。
