Kocić G, Vlahović P, Dordević V, Bjelaković G, Koraćević D, Savić V
Institute of Biochemistry, Nis, Serbia, Yugoslavia.
Arch Physiol Biochem. 1995 Dec;103(6):715-9. doi: 10.3109/13813459508998140.
Hepatocytes are affected by many cytokines and growth factors during liver regeneration. In regenerating rat liver cells cultures, liver cell growth factor (LCGF), hepatic stimulator substance (HSS), interleukin-1 beta (IL-1 beta), as well as their combination, were tested for their ability to activate the enzymes involved in purine metabolism. The enzymes tested were 5' nucleotidase, AMP deaminase, adenosine deaminase and xanthine oxidase. The cytokines alone or in combination, activated 5' nucleotidase and adenosine deaminase. Activity of AMP deaminase was stimulated by IL-1 beta associated with LCGF, HSS and IL-1 beta. Xanthine oxidase was stimulated by IL-1 beta but not with HSS and LCGF. Associated with IL-1 beta these two substances decreased its activity. A novel approach to the understanding of the mechanisms involved in the regulation of purine metabolism during liver regeneration, is proposed.
在肝脏再生过程中，肝细胞会受到多种细胞因子和生长因子的影响。在再生大鼠肝细胞培养物中，对肝细胞生长因子（LCGF）、肝刺激物质（HSS）、白细胞介素-1β（IL-1β）以及它们的组合激活参与嘌呤代谢的酶的能力进行了测试。所测试的酶包括5'核苷酸酶、AMP脱氨酶、腺苷脱氨酶和黄嘌呤氧化酶。单独或组合的细胞因子激活了5'核苷酸酶和腺苷脱氨酶。IL-1β与LCGF、HSS和IL-1β联合可刺激AMP脱氨酶的活性。黄嘌呤氧化酶受到IL-1β的刺激，但不受HSS和LCGF的刺激。与IL-1β相关，这两种物质降低了其活性。本文提出了一种理解肝脏再生过程中嘌呤代谢调节机制的新方法。
