Chen S, Li W, Yang Y
Shanghai Institute of Cardiovascular Diseases, Shanghai, Medical University.
Zhonghua Yi Xue Za Zhi. 1995 Oct;75(10):592-3, 638.
To examine the possible role of HCMV in the etiology of atherosclerosis, we searched for the presence of HCMV DNA in arterial vascular tissues by in situ hybridization. Biotin labelled HCMV DNA probe was used on specimens of arterial wall taken from patients with clinically significant atherosclerosis. Evidence for the presence of HCMV DNA was obtained in 13 specimens out of 32 atherosclerosis specimens. In contrast, only 1 specimen was positive in the control group (Total 15 specimens of normal arterial and venous wall), suggesting that there is a close relationship between human cytomegalovirus infection and atherosclerosis development. The results also showed that the positive hybridization signals were mainly observed in the nuclei of smooth muscular cells, rarely found in outer layer of arterial walls, cell membrane and cytoplasm. It was indicating that HCMV infection might be an etiological agent in the development of atherosclerosis.
为了研究人巨细胞病毒（HCMV）在动脉粥样硬化病因学中可能的作用，我们通过原位杂交技术检测动脉血管组织中HCMV DNA的存在情况。将生物素标记的HCMV DNA探针用于取自具有临床显著意义的动脉粥样硬化患者的动脉壁标本。在32例动脉粥样硬化标本中，有13例获得了HCMV DNA存在的证据。相比之下，对照组（共15例正常动脉和静脉壁标本）中只有1例呈阳性，这表明人巨细胞病毒感染与动脉粥样硬化的发展密切相关。结果还显示，阳性杂交信号主要出现在平滑肌细胞核中，在动脉壁外层、细胞膜和细胞质中很少发现。这表明HCMV感染可能是动脉粥样硬化发展的一个病因。
