在动脉粥样硬化斑块和未受影响的搭桥移植物中检测单纯疱疹病毒、巨细胞病毒和EB病毒DNA。

Detection of herpes simplex virus, cytomegalovirus and Epstein-Barr virus DNA in atherosclerotic plaques and in unaffected bypass grafts.

作者信息

Ibrahim Ali I, Obeid Michel T, Jouma Muhidien J, Moasis Ghassan A, Al-Richane Wael L, Kindermann Ingrid, Boehm Michael, Roemer Klaus, Mueller-Lantzsch Nikolaus, Gärtner Barbara C

机构信息

Department of Clinical Chemistry and Microbiology, University of Damascus, Syria.

出版信息

J Clin Virol. 2005 Jan;32(1):29-32. doi: 10.1016/j.jcv.2004.06.010.

DOI:10.1016/j.jcv.2004.06.010

PMID:15572003

Abstract

BACKGROUND

Herpes virus infections are suspected to be involved in the pathogenesis of atherosclerosis.

OBJECTIVE AND METHOD

Viral DNA of herpes simplex virus (HSV), Epstein-Barr virus (EBV) and cytomegalovirus (CMV) was analyzed by real-time PCR on 48 biopsies from atherosclerotic plaques extracted by end-arterectomy (46 coronary arteries, 2 carotid arteries), and in tissue from non-atherosclerosis vessels from the same patient as controls (23 internal mammary arteries, 43 saphenous veins).

RESULTS

HSV-1 DNA was detected significantly more frequently in plaques (35%) than in control veins (9%, P = 0.006). However, the frequency of HSV-1 DNA detection in the internal mammary artery grafts was as high as in plaques (22%, P = 0.28). CMV and EBV DNA were exclusively found in plaques but not in controls, with 10% for CMV (P = 0.06 versus veins, P = 0.17 versus graft arteries) and 2% for EBV (P = 1.0), respectively. HSV-2 was neither detected in plaques nor in controls. Herpes viral DNA was significantly associated only with arterial hypertension but not with other classical risk factors (P = 0.02), in accordance with the hypothesis that herpes viral infection may alter the vessel wall.

CONCLUSION

We conclude that herpes viral infections may have a role in atherosclerosis and that the presence of herpes viral DNA in the grafts used for bypass surgery might constitute a potential risk for atherosclerosis or restenosis.

摘要

背景

怀疑疱疹病毒感染参与动脉粥样硬化的发病机制。

目的与方法

采用实时聚合酶链反应（PCR）分析单纯疱疹病毒（HSV）、爱泼斯坦-巴尔病毒（EBV）和巨细胞病毒（CMV）的病毒DNA，样本来自48例通过动脉内膜切除术获取的动脉粥样硬化斑块活检组织（46例冠状动脉，2例颈动脉），以及来自同一患者的非动脉粥样硬化血管组织作为对照（23例乳内动脉，43例大隐静脉）。

结果

HSV-1 DNA在斑块中的检出频率（35%）显著高于对照静脉（9%，P = 0.006）。然而，HSV-1 DNA在乳内动脉移植物中的检出频率与斑块中的一样高（22%，P = 0.28）。CMV和EBV DNA仅在斑块中发现，对照中未发现，CMV为10%（与静脉相比，P = 0.06；与移植物动脉相比，P = 0.17），EBV为2%（P = 1.0）。HSV-2在斑块和对照中均未检出。疱疹病毒DNA仅与动脉高血压显著相关，与其他经典危险因素无关（P = 0.02），这与疱疹病毒感染可能改变血管壁的假说一致。