Hendrix M G, Salimans M M, van Boven C P, Bruggeman C A
University of Limburg, Department of Medical Microbiology, Maastricht, The Netherlands.
Am J Pathol. 1990 Jan;136(1):23-8.
The presence of cytomegalovirus (CMV) nucleic acids was demonstrated in arterial walls of patients with grade III and with maximally grade I atherosclerosis by dot blot and in situ DNA hybridization and by polymerase chain reaction (PCR) using probes and primers derived from immediate early (IE) and late (L) genomic regions. The presence of the complete viral genome could be demonstrated by both dot blot DNA hybridization and PCR. IE mRNA but not L mRNA could be demonstrated by in situ DNA hybridization, indicating the presence of latent CMV in the human arterial wall. By PCR 90% of the samples obtained from atherosclerotic patients were shown to contain viral nucleic acids as compared to 53% of patients with maximally grade I atherosclerosis, thus substantiating a role for this virus in the pathogenesis of atherosclerosis.
通过斑点印迹法、原位DNA杂交以及使用源自即刻早期（IE）和晚期（L）基因组区域的探针和引物进行聚合酶链反应（PCR），在III级和最大为I级动脉粥样硬化患者的动脉壁中证实了巨细胞病毒（CMV）核酸的存在。通过斑点印迹DNA杂交和PCR均可证实完整病毒基因组的存在。通过原位DNA杂交可证实存在IE mRNA，但不存在L mRNA，这表明人动脉壁中存在潜伏性CMV。通过PCR检测发现，与最大为I级动脉粥样硬化患者的53%相比，从动脉粥样硬化患者获得的样本中有90%含有病毒核酸，从而证实了这种病毒在动脉粥样硬化发病机制中的作用。
