Tsang S, Saier M H
Department of Biology, University of California at San Diego, La Jolla 92093-0116, USA.
J Comput Biol. 1996 Spring;3(1):185-90. doi: 10.1089/cmb.1996.3.185.
We describe a simple, flexible program (AAD) with a primary function of depicting the distribution of aliphatic and aromatic amino acid residues along the linear aligned sequence of a family of homologous proteins and a secondary function of depicting the distribution of all amino acids along the same linear sequence. The program is used to examine the distribution of aromatic versus aliphatic residues in representative well-characterized families of polytopic membrane proteins. Many but not all such protein families are shown to exhibit a predominance of aliphatic residues in the central regions of their transmembrane spanners but a predominance of aromatic residues at the peripheries of their spanners. We propose that this distribution stabilizes the hydrophobic-hydrophilic interface and renders the centers of these integral membrane proteins more fluid than their peripheries.
我们描述了一个简单灵活的程序（AAD），其主要功能是描绘脂肪族和芳香族氨基酸残基沿同源蛋白质家族线性比对序列的分布，次要功能是描绘所有氨基酸沿同一线性序列的分布。该程序用于研究多跨膜蛋白代表性特征明确家族中芳香族与脂肪族残基的分布。许多但并非所有此类蛋白质家族在其跨膜区域的中心区域显示出脂肪族残基占优势，而在其跨膜区域的周边则显示出芳香族残基占优势。我们提出，这种分布稳定了疏水 - 亲水界面，并使这些整合膜蛋白的中心比其周边更具流动性。
