Belen'kaia I M, Zarubina I L, Spitkovskaia L V
Arkh Anat Gistol Embriol. 1977;72(1):72-6.
The work presents a new installation and a method for intravital investigation of microcirculation in man. The installation is a modification of the contact fluorescent microscope MLK-1 produced by the Leningrad Optico-Mechanical Amalgamation which consists of the introduction of a polarization light-dividing prism into the optical scheme of the microscope for examination of the object in polarized falling rays and of substitution of the illumination source of the microscope (lamp KLM9-75) for the xenon lamp DKSSh-120, working both in the standard and given impulse regimen. The latter opens opportunties for determination of the blood flow rate in capillaries. The determination principle is that the effect of bloodflow "stop" is observed in the case of "coincidence" of the bloodflow in the capillary and the lamp modulation rate. The mucouse membrane of the oral cavity (cheek) was used as the object for investigation. It has been shown that its microcirculatory bed in normality is represented by capillary loops in the shape of a hair-pin having the diameter of 2;92--7,74 mkm. Their amount in the view field fluctuates between 8 and 20. The average blood flow rate in the capillaries under study is 0,4 mm/sec.
这项工作展示了一种用于人体微循环活体研究的新装置和方法。该装置是对列宁格勒光学机械联合企业生产的MLK - 1型接触式荧光显微镜的改进，改进内容包括在显微镜的光学系统中引入一个偏振分光棱镜，以便在偏振落射光下检查物体，并用氙灯DKSSh - 120替代显微镜的照明光源（KLM9 - 75灯），氙灯可在标准和给定脉冲模式下工作。后者为测定毛细血管中的血流速度提供了机会。测定原理是，当毛细血管中的血流与灯的调制速率“重合”时，会观察到血流“停止”的效应。口腔（脸颊）黏膜被用作研究对象。结果表明，在正常情况下，其微循环床由发夹状的毛细血管袢组成，直径为2.92 - 7.74微米。视野中它们的数量在8到20之间波动。所研究的毛细血管中的平均血流速度为0.4毫米/秒。
