Fujioka S, Tsukamoto S, Oshida S, Shimamura M, Nagoya T
Department of Legal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Nihon Arukoru Yakubutsu Igakkai Zasshi. 1996 Feb;31(1):67-80.
Male rats of the Wistar strain were divided 4 groups, and give a liquid diet of control feed, bezafibrate (150 mg/kg), ethanol, and ethanol plus bezafibrate for 5 week. The effect of bezafibrate supplementation on rats fed ethanol was examined in terms of the fatty acid composition of the phospholipids in the erythrocyte membrane. In the phospholipids profiles of erythrocyte membranes, PI was significantly decreased. The decrease in PI caused by bezafibrate appeared to substantially affect the membrane and consequently lead to changes in the membrane anchor. In the fatty acid composition of the PC, C20: 4 was significantly decreased in the group receiving alcohol (p < 0.05) but increased in the groups receiving bezafibrate (p < 0.05). In the fatty acid composition of the PE, C16: 0 was significantly increased in the three groups when compared with the control, and C20: 4 was decreased in the alcohol group (p < 0.05). In the fatty acid of SM and PI, C20: 4 was decreased and C18: 0 increased in the alcohol group. In the PS, C14: 0 was increased in the alcohol group, and decreased in the alcohol plus bezafibrate group (p < 0.01). The levels of arachidonic acid in the total fatty acids that constituted the membrane phospholipids were decreased in the rats given ethanol (p < 0.05). However, arachidonic acid in the group of bezafibrate supplementation on rats fed ethanol were elevated in comparison with the alcohol group (p < 0.05). With decreasing arachidonic acid as a marker of alcohol tissue injury following chronic alcohol intake, the effects of bezafibrate supplementation appear to contribute to membrane fluidity by altering the biochemical flexibility of the membrane.
将Wistar品系的雄性大鼠分为4组，分别给予对照饲料、苯扎贝特（150毫克/千克）、乙醇以及乙醇加苯扎贝特的流质饮食，持续5周。从红细胞膜磷脂的脂肪酸组成方面，研究了补充苯扎贝特对喂食乙醇大鼠的影响。在红细胞膜的磷脂谱中，磷脂酰肌醇（PI）显著降低。苯扎贝特引起的PI降低似乎对膜产生了实质性影响，进而导致膜锚定的变化。在磷脂酰胆碱（PC）的脂肪酸组成中，接受酒精的组中二十碳四烯酸（C20:4）显著降低（p<0.05），但接受苯扎贝特的组中该脂肪酸增加（p<0.05）。在磷脂酰乙醇胺（PE）的脂肪酸组成中，与对照组相比，三组中的十六烷酸（C16:0）均显著增加，而酒精组中的C20:4降低（p<0.05）。在鞘磷脂（SM）和PI的脂肪酸中，酒精组中C20:4降低，十八烷酸（C18:0）增加。在磷脂酰丝氨酸（PS）中，酒精组中十四烷酸（C14:0）增加，而酒精加苯扎贝特组中该脂肪酸降低（p<0.01）。给予乙醇的大鼠中，构成膜磷脂的总脂肪酸中的花生四烯酸水平降低（p<0.05）。然而，与酒精组相比，补充苯扎贝特的大鼠组中花生四烯酸升高（p<0.05）。以花生四烯酸降低作为慢性酒精摄入后酒精组织损伤的标志物，补充苯扎贝特的作用似乎通过改变膜的生化柔韧性来促进膜流动性。
