Yang S, Wang M, You W
PLA General Hospital, Beijing.
Zhonghua Zhong Liu Za Zhi. 1995 Nov;17(6):415-7.
Immunohistochemistry (ABC method) and in situ hybridization (DNA-RNA) were used to detect c-myc and p53 gene expression in tissues of human HCC and nearby non-tumorous liver (NT) from 23 patients. The results showed that the positive rates of P62c-myc were 87% (20/23) in HCC and 91% (21/23) in NT. The positive rates of P53 protein were 39% (9/23) in HCC as well as in NT. The positive rates of c-myc and p53 mRNA were 70% (16/23) and 56% (13/23) in HCC and NT respectively. The expression of c-myc and p53 at protein level was significantly correlated with that at mRNA level. These observations suggest a close association of c-myc and p53 gene overexpression with hepatocarcinogenesis. Immunohistochemistry (ABC method) on section of paraffin embedded tissue is a reliable method for detecting c-myc and p53 gene expression in HCC.
采用免疫组织化学（ABC法）和原位杂交（DNA-RNA法）检测23例人类肝癌组织及其癌旁非肿瘤肝脏组织（NT）中c-myc和p53基因的表达。结果显示，肝癌组织中P62c-myc阳性率为87%（20/23），癌旁非肿瘤肝脏组织中为91%（21/23）。肝癌组织和癌旁非肿瘤肝脏组织中P53蛋白阳性率均为39%（9/23）。肝癌组织和癌旁非肿瘤肝脏组织中c-myc和p53 mRNA阳性率分别为70%（16/23）和56%（13/23）。c-myc和p53蛋白水平的表达与mRNA水平显著相关。这些观察结果提示c-myc和p53基因过表达与肝癌发生密切相关。石蜡包埋组织切片的免疫组织化学（ABC法）是检测肝癌中c-myc和p53基因表达的可靠方法。
