Wachtel S S, Sammons D, Manley M, Wachtel G, Twitty G, Utermohlen J, Phillips O P, Shulman L P, Taron D J, Müller U R, Koeppen P, Ruffalo T M, Addis K, Porreco R, Murata-Collins J, Parker N B, McGavran L
Department of Obstetrics and Gynecology, University of Tennessee, Memphis 38103, USA.
Hum Genet. 1996 Aug;98(2):162-6. doi: 10.1007/s004390050181.
Fetal blood cells can be recovered from the maternal circulation by charge flow separation (CFS), a method that obviates the risks associated with amniocentesis and chorionic villus sampling. By CFS, we processed blood samples from 13 women carrying male fetuses, 2 carrying fetuses with trisomy 21, and 1 who had delivered a stillborn infant with trisomy 18. On average more than 2000 fetal nucleated red blood cells were recovered per 20-ml sample of maternal blood. Recovery of fetal cells was confirmed by fluorescence in situ hybridization with probes for chromosomes Y, 18 and 21. After culturing of CFS-processed cells, amplification by the polymerase chain reaction revealed Y-chromosomal DNA in clones from four of six women bearing male fetuses, but not in clones from three women bearing female fetuses.
胎儿血细胞可通过电荷流分离法（CFS）从母体循环中获取，该方法可避免与羊膜穿刺术和绒毛取样相关的风险。通过CFS，我们对13名怀有男性胎儿的女性、2名怀有21三体胎儿的女性以及1名分娩出18三体死产婴儿的女性的血样进行了处理。每20毫升母体血液样本平均可获取2000多个胎儿有核红细胞。通过使用针对Y、18和21号染色体的探针进行荧光原位杂交，确认了胎儿细胞的获取。在对经CFS处理的细胞进行培养后，聚合酶链反应扩增显示，在6名怀有男性胎儿的女性中的4名的克隆中检测到Y染色体DNA，但在3名怀有女性胎儿的女性的克隆中未检测到。
