Karakurt Ilkay, Ozaltin Kadir, Vesela Daniela, Lehocky Marian, Humpolíček Petr, Mozetič Miran
Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Nam. T.G.M. 5555, 76001 Zlín, Czech Republic.
Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 76001 Zlín, Czech Republic.
Polymers (Basel). 2019 Jul 15;11(7):1186. doi: 10.3390/polym11071186.
Polylactic acid (PLA) is one of the most produced polymeric materials, due to its exceptional chemical and mechanical properties. Some of them, such as biodegradability and biocompatibility, make them attractive for biomedical applications. Conversely, the major drawback of PLA in the biomedical field is their vulnerability to bacterial contamination. This study focuses on the immobilization of saccharides onto the PLA surface by a multistep approach, with the aim of providing antibacterial features and evaluting the synergistic effect of these saccharides. In this approach, after poly (acrylic acid) (PAA) brushes attached non-covalently to the PLA surface via plasma post-irradiation grafting technique, immobilization of glucosamine (GlcN) and chondroitin sulfate (ChS) to the PAA brushes was carried out. To understand the changes in surface properties, such as chemical composition, surface topography and hydrophilicity, the untreated and treated PLA films were analyzed using various characterization techniques (contact angle, scanning electron microscopy, X-ray photoelectron spectroscopy). In vitro cytotoxicity assays were investigated by the methyl tetrazolium test. The antibacterial activity of the PLA samples was tested against Escherichia coli and Staphylococcus aureus bacteria strains. Plasma-treated films immobilized with ChS and GlcN, separately and in combination, demonstrated bactericidal effect against the both bacteria strains and also the results revealed that the combination has no synergistic effect on antibacterial action.
聚乳酸（PLA）是产量最高的聚合物材料之一，因其具有优异的化学和机械性能。其中一些特性，如生物可降解性和生物相容性，使其在生物医学应用中具有吸引力。相反，PLA在生物医学领域的主要缺点是其易受细菌污染。本研究重点通过多步方法将糖类固定在PLA表面，旨在提供抗菌特性并评估这些糖类的协同效应。在该方法中，通过等离子体后辐照接枝技术使聚（丙烯酸）（PAA）刷非共价连接到PLA表面后，将氨基葡萄糖（GlcN）和硫酸软骨素（ChS）固定到PAA刷上。为了解表面性质的变化，如化学成分、表面形貌和亲水性，使用各种表征技术（接触角、扫描电子显微镜、X射线光电子能谱）对未处理和处理过的PLA薄膜进行分析。通过甲基噻唑蓝试验研究体外细胞毒性测定。测试了PLA样品对大肠杆菌和金黄色葡萄球菌菌株的抗菌活性。分别和组合固定有ChS和GlcN的等离子体处理薄膜对两种细菌菌株均表现出杀菌作用，结果还表明该组合在抗菌作用上没有协同效应。
