Gundlach B R, Wiesmüller K H, Junt T, Kienle S, Jung G, Walden P
Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany.
J Immunol Methods. 1996 Jun 10;192(1-2):149-55. doi: 10.1016/0022-1759(96)00040-3.
A new approach to T cell epitope determination is presented. Critical amino acids for the induction of cytotoxic T cell responses were identified using synthetic peptide libraries with single defined sequence positions combined with randomized sequence positions. Sequences for potential T cell epitopes were deduced from scan profiles using combinations of the active amino acids. Highly potent epitopes for cytotoxic T lymphocytes were obtained. Epitopes defined by this approach are, as shown in this communication, not necessarily the natural epitopes and, therefore, were named synthetic epitopes. They can serve effectively for the development of vaccines or for the determination of T cell receptor antagonists.
提出了一种确定T细胞表位的新方法。使用具有单个确定序列位置和随机序列位置相结合的合成肽文库，鉴定了诱导细胞毒性T细胞反应的关键氨基酸。利用活性氨基酸的组合从扫描图谱中推导潜在T细胞表位的序列。获得了高效的细胞毒性T淋巴细胞表位。如本通讯所示，通过这种方法确定的表位不一定是天然表位，因此被命名为合成表位。它们可有效地用于疫苗开发或T细胞受体拮抗剂的确定。
