al-Obeidi F, Hruby V J, Sawyer T K
Selectide Research Center, a Subsidiary of Hoechst-Marion-Roussel, Tucson, AZ, USA.
Mol Biotechnol. 1998 Jun;9(3):205-23. doi: 10.1007/BF02915794.
Various techniques for generation of peptide and peptidomimetic libraries are summarized in this article. Multipin, tea bag, and split-couple-mix techniques represent the major methods used to make peptides and peptidomimetics libraries. The synthesis of these libraries were made in either discrete or mixture format. Peptides and peptidomimetics combinatorial libraries were screened to discover leads against a variety of targets. These targets, including bacteria, fungus, virus, receptors, and enzymes were used in the screening of the libraries. Discovered leads can be further optimized by combinatorial approaches.
本文总结了生成肽和拟肽文库的各种技术。多针、茶包和拆分偶联混合技术是用于制备肽和拟肽文库的主要方法。这些文库的合成采用离散或混合形式。对肽和拟肽组合文库进行筛选以发现针对各种靶标的先导化合物。这些靶标包括细菌、真菌、病毒、受体和酶，用于文库的筛选。发现的先导化合物可通过组合方法进一步优化。
