Corry D B, Ampel N M, Christian L, Locksley R M, Galgiani J N
Infectious Diseases Division, University of California, San Francisco, USA.
J Infect Dis. 1996 Aug;174(2):440-3. doi: 10.1093/infdis/174.2.440.
The production and mRNA expression of the cytokines interferon-gamma (IFN-gamma) and interleukin (IL)-4, -10, and -12 by peripheral blood mononuclear cells (PBMC) after incubation with the coccidioidal antigen toluene spherule lysate (TSL) from various subjects were measured. The IFN-gamma concentration in PBMC supernatants incubated for 72 h from 8 subjects with disseminated coccidioidomycosis was significantly less than that from 7 healthy, coccidioidal-immune subjects (P = .015). No differences were seen among the subject groups in the concentrations of IL-4, -10, or -12. By use of competitive polymerase chain reaction, PBMC from subjects with disseminated coccidioidomycosis also expressed less mRNA for IFN-gamma and IL-12 than did cells from healthy, immune subjects. These data suggest that patients with disseminated coccidioidomycosis have a diminished T helper lymphocyte type 1 response.
测定了来自不同受试者的外周血单个核细胞（PBMC）与球孢子菌抗原甲苯球囊裂解物（TSL）孵育后细胞因子干扰素-γ（IFN-γ）、白细胞介素（IL）-4、-10和-12的产生及mRNA表达。8例播散性球孢子菌病患者的PBMC在孵育72小时后的上清液中IFN-γ浓度显著低于7例健康的、对球孢子菌免疫的受试者（P = 0.015）。各受试者组间IL-4、-10或-12的浓度未见差异。通过竞争性聚合酶链反应，播散性球孢子菌病患者的PBMC中IFN-γ和IL-12的mRNA表达也低于健康免疫受试者的细胞。这些数据表明，播散性球孢子菌病患者的1型辅助性T淋巴细胞反应减弱。
