Hua J, Liao M J, Rashidbaigi A
Interferon Sciences, Inc., New Brunswick, New Jersey 08901, USA.
J Leukoc Biol. 1996 Jul;60(1):125-8. doi: 10.1002/jlb.60.1.125.
Human peripheral blood leukocytes (hPBL) are a rich source of natural leukocyte interferon (IFN-alpha) when treated with Sendai virus. Sendai virus treatment of hPBL will also result in significant production of several chemokines and cytokines such as macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, RANTES, tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and IL-8, in a time-dependent way. A significant amount of MCP-1 is constitutively produced in overnight culture of leukocytes. The most abundant cytokine is IFN-alpha, which is induced to its maximum level approximately 11-15 h after addition of Sendai virus. The amount of IFN-alpha induced at 15 h after Sendai virus treatment is more than 16-fold higher than those of MIP-1alpha, MIP-1beta, and RANTES. IFN-alpha is also induced more than 60-fold higher than TNF-alpha and IL-8. The amount of IL-6 induced is approximately 400-fold less than IFN-alpha. Limited amounts of other cytokines such as IL-1alpha, IL-1beta, macrophage colony-stimulating factor, TNF-beta, and IFN-gamma are also induced in Sendai virus-treated hPBL. No measurable amount of granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor, leukemia inhibitory factor, IL-2, IL-3, IL-4, IL-5, IL-7, IL-10, IL-11, or IL-12 was induced in the supernatant of Sendai virus-treated hPBL.
人外周血白细胞（hPBL）在用仙台病毒处理后是天然白细胞干扰素（IFN-α）的丰富来源。用仙台病毒处理hPBL还会导致几种趋化因子和细胞因子以时间依赖性方式大量产生，如巨噬细胞炎性蛋白-1α（MIP-1α）、MIP-1β、调节激活正常T细胞表达和分泌的趋化因子（RANTES）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和IL-8。在白细胞过夜培养中组成性产生大量单核细胞趋化蛋白-1（MCP-1）。最丰富的细胞因子是IFN-α，在加入仙台病毒后约11 - 15小时诱导至最高水平。仙台病毒处理15小时后诱导的IFN-α量比MIP-1α、MIP-1β和RANTES高16倍以上。IFN-α的诱导量也比TNF-α和IL-8高60倍以上。诱导的IL-6量比IFN-α少约400倍。在仙台病毒处理的hPBL中也诱导产生少量其他细胞因子，如IL-1α、IL-1β、巨噬细胞集落刺激因子、TNF-β和IFN-γ。在仙台病毒处理的hPBL上清液中未诱导出可测量量的粒细胞-巨噬细胞集落刺激因子、粒细胞集落刺激因子、白血病抑制因子、IL-2、IL-3、IL-4、IL-5、IL-7、IL-10、IL-1或IL-12。
