Bhatia K, Raj A, Guitierrez M I, Judde J G, Spangler G, Venkatesh H, Magrath I T
Lymphoma Biology Section, Pediatric Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Oncogene. 1996 Jul 4;13(1):177-81.
We have examined sequence variations in the EBNA-1 protein of EBV in normal peripheral blood lymphocytes (PBL) and Burkitt's lymphomas (BL). We find two EBNA-1 strains P (prototype) and V (variant) which differ by 15 amino acids. Each strain has two subtypes defined by the amino acid at position 487 (P-ala, P-thr, V-pro and V-leu). In PBLs from 32 normal individuals, up to three of these subtypes were found in each sample, but the V strain did not occur in the absence of P strain viruses, nor was the V-leu subtype ever observed in normal PBL. In BLs only a single subtype was observed in each tumor. The P-thr and V-leu subtypes were more frequently seen than the P-ala and V-pro subtypes, which occurred in only two and one of the 36 tumor samples respectively. The P-thr was the most commonly observed subtype in peripheral blood of both American and African lymphocytes as well as in African tumors. However, in 11 of 12 American tumors, the EBNA-1 subtype was V-leu. These data indicate that some EBNA-1 subtypes are more likely to lead to oncogenesis, and one subtype, V-leu, appears only to occur in tumors.
我们检测了EB病毒（EBV）的EBNA-1蛋白在正常外周血淋巴细胞（PBL）和伯基特淋巴瘤（BL）中的序列变异。我们发现了两种EBNA-1毒株，P（原型）和V（变异型），它们相差15个氨基酸。每种毒株有两个亚型，由第487位氨基酸定义（P-ala、P-thr、V-pro和V-leu）。在32名正常个体的PBL中，每个样本中发现多达三种这些亚型，但在没有P毒株病毒的情况下不会出现V毒株，在正常PBL中也从未观察到V-leu亚型。在BL中，每个肿瘤中仅观察到单一亚型。P-thr和V-leu亚型比P-ala和V-pro亚型更常见，P-ala和V-pro亚型分别仅出现在36个肿瘤样本中的两个和一个样本中。P-thr是在美国和非洲淋巴细胞外周血以及非洲肿瘤中最常观察到的亚型。然而，在12个美国肿瘤中的11个中，EBNA-1亚型是V-leu。这些数据表明，一些EBNA-1亚型更有可能导致肿瘤发生，并且一种亚型V-leu似乎仅出现在肿瘤中。
