Bezerra J de M, Vale A V, Lobato Filho J C, Martins S F, Albarelli A L, Freire S de J, de Oliveira E G, Longo J C
Departamento de Patologia, Universidade Federal do Maranhão, São Luís, MA.
Rev Soc Bras Med Trop. 1996 May-Jun;29(3):245-50. doi: 10.1590/s0037-86821996000300005.
With previously defined criteria of inclusion and previous consent, twenty six consecutive patients (19 to 64 years old), with upper digestive symptoms, were submitted to endoscopy, with biopsy, constant of eight samples of the antropyloric region (four of the anterior aspect and four of the posterior aspect). Two samples were been for culture; two for free urease test, two for smears; all gathered in adequate transport medium over refrigeration. Two samples immersed in formaline to 10% for histopathologic exam. 25/26 (96%) of the patients showed infection by H. pylori by means of one or more methods utilized. In 16/26 (61%), alterations were observed in endoscopy (gastric inflammation in eleven, peptic ulcer in two and ulcer scars in three cases). Of the patients with endoscopic gastric inflammation, presented positive as well as all (100%) bearers of scar or peptic ulcer. A close relationship was observed between the presence of H. pylori and chronic gastric inflammation 24/25 (96%). Histopathologic slices stained by hematoxilin-eosin was the test of highest diagnostic sensitivity 24/25 (96%), followed by urease test 23%25 (92%), stained smears 19/25 (76%) and culture 18/25 (72%). Our conclusion is that the prevalence of gastric infection for H. pylori in symptomatic patients is high, correlated with chronic gastric inflammation and ulcers. Histopathologic slices stained by hematoxilin-eosin and free urease test are the most sensitive indicators of the presence of H. pylori. This study should proceed for further elucidation of questions realised and include a control group of symptomatics individuals paired for sex and age.
根据先前确定的纳入标准和事先获得的同意，26例连续的患者（年龄在19至64岁之间），有上消化道症状，接受了内镜检查并取活检，从胃幽门区域取8个样本（前壁4个，后壁4个）。2个样本用于培养；2个用于游离尿素酶试验，2个用于涂片；所有样本均收集在合适的运输培养基中并冷藏。2个样本浸入10%的福尔马林中用于组织病理学检查。通过一种或多种使用的方法，25/26（96%）的患者显示感染幽门螺杆菌。16/26（61%）的患者在内镜检查中观察到病变（11例为胃炎，2例为消化性溃疡，3例为溃疡瘢痕）。在内镜下有胃炎的患者中，所有有瘢痕或消化性溃疡的患者（100%）也呈阳性。观察到幽门螺杆菌的存在与慢性胃炎之间有密切关系，24/25（96%）。苏木精-伊红染色的组织病理学切片是诊断敏感性最高的检测方法，24/25（96%），其次是尿素酶试验23/25（92%），染色涂片19/25（76%）和培养18/25（72%）。我们的结论是，有症状患者中幽门螺杆菌胃部感染的患病率很高，与慢性胃炎和溃疡相关。苏木精-伊红染色的组织病理学切片和游离尿素酶试验是幽门螺杆菌存在的最敏感指标。本研究应继续进行，以进一步阐明所提出的问题，并纳入一个按性别和年龄配对的有症状个体的对照组。
