Lee S Y, Lee K P, Lim J W
Department of Natural Sciences, Catholic University Medical College, Seoul, Korea.
Thromb Haemost. 1996 Mar;75(3):466-70.
Fibrinogen has been detected in ME-180 human uterine cervix carcinoma cells, and synthesis of fibrinogen by ME-180 cells has been measured using [35S] L-methionine incorporation. Expression of mRNA for the B beta-chain of the fibrinogen in ME-180 cells has been identified by PCR-assisted mRNA amplification. The A alpha-, B beta-, and gamma-chains of fibrinogen synthesized by ME-180 cells were chemically and immunologically identical to those of plasma fibrinogen. These findings suggest that fibrinogen is present and synthesized in ME-180 human uterine cervix carcinoma cells, and that fibrinogen plays a role in these malignant tumor cells.
在ME-180人子宫颈癌细胞中已检测到纤维蛋白原，并且使用[35S]L-甲硫氨酸掺入法测定了ME-180细胞合成纤维蛋白原的情况。通过PCR辅助的mRNA扩增鉴定了ME-180细胞中纤维蛋白原Bβ链的mRNA表达。ME-180细胞合成的纤维蛋白原的Aα、Bβ和γ链在化学和免疫方面与血浆纤维蛋白原的链相同。这些发现表明纤维蛋白原存在于ME-180人子宫颈癌细胞中并在其中合成，并且纤维蛋白原在这些恶性肿瘤细胞中发挥作用。
